248 
PACIFIC SCIENCE, VoL XVII, April 1963 
TABLE 1 
Free and Protein-bound Amino Acids Present in Specimens of Mytilus edulis 
From Three Polluted Areas, One Nonpolluted Area, and Transferred Specimens 
ALANINE | 
GLYCINE 
GLUTAMIC ACID 
SERINE 
ASPARTIC ACID 
THREONINE 
LYSINE 
HISTIDINE 
ARGININE 
valine/phenylalanine 
LEUCINES 
CYSTINE 
CYSTEINE 
PROLINE 
TYROSINE 
METHIONINE 
asparagine/taurine 
UNKNOWNS 
Free Amino Acids 
Polluted areas 
L. A. 7 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
2 
L. A. 39 
X 
X 
X 
X 
X 
X 
X 
~x 
X 
X 
X 
X 
X 
X 
3 
L. B. 23 
X 
X 
X 
X 
x~ 
X 
X 
X 
X 
X 
X 
X 
X 
X 
1 
Free Amino Acids 
Nonpolluted area 
Tomales Bay 
X 
X 
X 
X 
X 
X 
X 
x~ 
x~ 
x~ 
X 
X 
X 
3 
Free Amino Acids 
Transferred 
To L. A. 39 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
To L. B. 23 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Hydrolysates 
L. A. 7 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
3 
L. A. 39 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
’x~ 
"x 
X 
X 
2 
L. B. 23 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
V 
~x 
x~ 
X 
X 
Tomales Bay 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
areas. After 10 days these specimens were re- 
moved and analyzed for free amino acid content 
in the manner described above. The terminal 
survival point for transferred animals occurred 
between 10 and 15 days. 
RESULTS 
Chromatograms made of the free amino acid 
extracts of specimens from clean water showed 
the presence of alanine, glycine, glutamic acid, 
serine, aspartic acid, threonine, lysine, histidine, 
arginine, valine, leucines, methionine, proline, 
plus three unidentified spots. 
Native specimens from L.A. 39, L.A. 7, and 
L.B. 23, all polluted areas, contained alanine, 
glycine, glutamic acid, serine, aspartic acid, thre- 
onine, lysine, histidine, arginine, valine, leucines, 
proline, cystine, and methionine, plus two un- 
identified spots in the case of L.A. 7 and three 
each on the chromatograms of L.A. 39 and L.B. 
23. (See Table 1.) 
The specimens taken from Tomales Bay and 
transferred to the polluted waters of L.A. 39 
and L.B. 23 contained well-defined spots of 
alanine, glycine, glutamic acid, serine, aspartic 
acid, threonine, lysine, histidine, arginine, valine, 
leucines, and taurine/asparagine. 
The hydrolysate of specimens from Tomales 
Bay contained alanine, glycine, glutamic acid, 
serine, aspartic acid, threonine, lysine, histidine, 
arginine, valine, methionine, leucines, cystine, 
and proline. 
