250 
PACIFIC SCIENCE, Vol. XVII, April 1963 
another) unidentifiable ninhydrin-sensitive 
spots. 
2. Specimens from polluted waters were ana- 
lyzed, after hydrolysis, for amino acid content of 
muscle tissue. Sixteen amino acids were identi- 
fied. Unidentifiable spots also occurred in speci- 
mens from two of the three locations. 
3. Specimens from nonpolluted water were 
treated in the same way. Thirteen free amino 
acids were identified. Cystine, present in free 
form from polluted water was lacking. Three 
unidentifiable spots were also present. Fourteen 
amino acids were identified from the protein 
hydrolysate. Cysteine and tyrosine, found in hy- 
drolysates of polluted water specimens, were 
lacking here. 
4. Specimens were transferred from clean 
water to polluted environments, left for 10 days, 
and then analyzed in the usual manner. Chro- 
matograms showed them to have 11 free amino 
acids in common with the others. Proline and 
methionine contained in the others were absent 
here. Cystine, present in those native to the 
polluted areas, was lacking. Taurine/ asparagine, 
not found in any of the others, appeared here. 
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