Photomicrographic Records — H siao, FUJII, and Fine 
325 
stopped by the fixed spacer at the end. A cul- 
ture slide containing the sample is placed on 
the plate glass with its length perpendicular to 
the long axis of the glass toppiece and directly 
under the low power objective of the micro- 
scope. In our experiments a glass or aluminum 
ring ^4-inch I. D. and 5/32-inch high is 
mounted in the center of a 1- X 3 -inch micro 
slide with epoxy cement to serve as a culture 
cell. When the culture slide has been centered, 
a piece of metal or glass is placed snugly against 
its right side and fixed on the plate glass with 
masking tape to serve as a slide end-stop. To 
facilitate lining up the other culture slides on 
the left side of this extreme right one the 
1-inch wide space between the 28-inch-long 
aluminum frame on the side nearest the oper- 
ator and the culture slide is filled with another 
1 X 3 -inch slide at right angles to the end- 
stop (see Fig. 2 C). Other culture slides are then 
easily placed in a straight line on the left of the 
original one as shown in Figure 2C by push- 
ing them toward the right lower corner against 
the slide end-stop. After all the culture slides 
are placed in a straight line from right to left, 
the microscope-carrying cart is moved toward 
the left by drawing it along the track and the 
1-inch-wide aluminum spacers are placed be- 
tween the cart and the end piece on the plat- 
form, the number of spacers used being equal 
to the number of culture slides. As both the 
spacers and the culture slides are 1-inch wide 
the slide on the extreme left will be centered 
directly under the microscope. The culture slides 
and spacers are numbered correspondingly, both 
starting with No. 1 on the left and ending 
with the highest number on the right. 
A photograph of the assembled device with 
the photomicrographic instrument in place is 
shown in Figure 3. 
To take photomicrographs with this device, 
a camera is mounted on the microscope, and 
the specimens in the culture cell brought into 
view. Usually, before the first exposure is made, 
the specimens are centered and arranged on 
each slide, using a glass needle, so that a large 
number of individuals can be observed in each 
microscope field. Once the specimens are prop- 
erly arranged, no other manipulation is needed, 
and they will stay in place throughout the 
course of the experiment. After the specimens 
Fig. 3. Photograph of device, with camera, micro- 
scope, and illuminator in place. 
are brought into sharp focus, a photograph is 
taken with the time of exposure and light in- 
tensity adjusted by the usual method for photo- 
graphic work (Shillaber, 1944). To take a 
photograph of the second culture, the first 
spacer on the left is removed, the microscope 
pushed 1 inch toward the right tightly against 
the remaining spacers, the specimens brought 
into sharp focus, and a second frame of the film 
exposed. This procedure is repeated until the 
microscope-carrying cart is against the stopping 
spacer on the extreme right of the platform, 
and each culture has been photographed. 
To start the second series of photomicro- 
graphs, the microscope is moved to the left, all 
the aluminum spacers replaced on the plat- 
form, maintaining their original order, and the 
cart pushed tightly against them. This auto- 
matically places the microscope objective over 
the center of the first culture. After a predeter- 
mined time lapse, the process of photomicrog- 
raphy described above is repeated, taking a sec- 
ond photograph of each one of the cultures, 
covering, in each case, exactly the same field 
as in the first run. The exact time of day a 
photograph is taken is noted down, and later 
