120 
PACIFIC SCIENCE, VoL VIII, April, 1954 
spermatocytes and spermatids, certain cells 
containing strongly chromatophoric nuclei 
arise from the germinal epithelium and be- 
come widely dispersed throughout the follicle. 
These sustentacular cells probably aid the 
spermatids in their metamorphoses into 
spermatozoa. 
Each group of spermatozoa seen in the 
collecting tubule is the extruded contents of 
a single follicle. This extrusion is caused by 
the production of a new group of primary 
spermatocytes filling the follicle. A secretion 
from the epithelial cells of the tubule sur- 
rounds each group of spermatozoa. As this 
material traverses the proximal portion of the 
vas deferens, a secretion from the undifferen- 
tiated epithelial cells surrounds the sperm 
mass and forms the spermatophoric sheath. 
No typhlosole-like structure is present in 
the enlarged vas deferens, but epithelial cells 
secrete a matrix which envelops the sperma- 
tophoric sheath. That spermatophoric wall 
and matrix are distinct secretions is attested 
both by the diastem which separates them 
and by the greater solubility of the matrix 
in 0.1 N KOH. 
Although neither naturally occurring sper- 
matophores nor the process of fertilization 
was actually observed, the facts that the ma- 
trix hardens and that the seminal receptacle 
does not open internally strongly suggest 
mechanical liberation of the spermatozoa and 
subsequent external fertilization. 
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