124 
PACIFIC SCIENCE, Vol. VIII, April, 1954 
Key to 
shading 
10-20 
>20 fTTTTTTm <75 
Fig. 4. Charts showing the distribution and approximate numbers of Kitteriella picteti, per 1,000 cubic meters 
of water filtered. Isotherms in degrees centigrade. 
is no problem, but in specimens in which the 
muscles are not easily seen separation of the 
species is complicated. The best solution to 
the problem yet found is staining the muscles. 
Eor the purpose of this "identification stain- 
ing,” Rose Bengal has been found very useful 
although not suitable for permanent prepara- 
tions. A stock solution of 0.1 per cent Rose 
Bengal may be added to a mixture of sea 
water and formalin in various amounts to give 
the intensity of staining desired, with no salt 
reaction. The main objection to the method 
is that the stain washes out very easily. 
The distribution of R. picteti during the pe- 
riod covered by this paper is shown in Figure 
4; it can be seen that the occurrence of the 
species is variable. The patchiness seen is typ- 
ical of distributions of "salps” and especially 
true of those which do not occur in large 
numbers and are not extremely common. This 
form appears to be much more tolerant of 
cold water than its relative R. amhoinensis. It 
has been taken in water with a temperature 
as low as 10 degrees at a depth of 10 meters. 
In the area examined, the lower limit of their 
range appears to be in the vicinity of this 
10-degree isotherm. 
REFERENCES 
Apstein, Carl. 1904. Salpes d’Amboine. Rep. 
Suisse de ZooL 12(3): 649-656. 
1906^. Salpen der Deutschen Tiefsee- 
Expedition. WissenschaftUche Ergebnisse der 
Deutsche Tie fsee -Expedition 12(3): 245-290. 
1906/^. Die Salpen der Deutschen Slid- ! 
polar-Expedition 1901-1903. Deutsche Sud- : 
polar -Expedition 155-203. ? 
Ihle, J. E. W. 1910. Die Thaliaceen (ein- i 
schliesslich pyrosomen) der Siboga Expedi- ; 
tion. Siboga Exped. 49(1): 1-55. t 
1912.Tunicata,SalpaeI, Desmomyaria. ^ 
In Das Tierreich [Ed. E. E. Schulze] Lief. ; 
32. xi+66 pp. R. Friedlander und Sohn, |; 
Berlin. | 
1935. Desmomyaria. In Handbuch der I 
Zoologie [Ed. Kiikenthal und Krumbach]. [ 
Vol. 5(2, 5), pp. 401-544. Walter de Gruy- 
ter & Co., Berlin und Leipzig. 
Komai, Taku. 1932. On some salps occur- 
ring in the vicinity of Seto, with remarks 
on the enatiomorphism found in some ag- |; 
gregate forms. Kyoto Univ., Col. Sci., Mem. \ 
8(1) series B; 65-80. j| 
Metcalf, Maynard M. 19I8. Contributions j 
to the biology of the Philippine Archipel- 1 
ago and its adjacent regions. D. S. Natl. \ 
Mus., Bui. 100(2) 2: 1-193. 
— 1919. Corrections to Metcalf I9I8. 
Science 50(1279): 19-20. j 
Ritter, W. E. 1906. Cyclosalpa retracta, a new 
salpoid from the coast of Japan. Annot. 
Zool. Jap. 6(1) : 1-5. ' 
Sewell, R. B. Seymour. 1926. The salps of j 
Indian seas. Indian Mus., Rec. 28(2) : 65-126. j 
Thompson, Harold. 1948. Pelagic tunicates : 
of Australia. 195 pp. Commonwealth Coun- 
cil for Scientific and Industrial Research 
[Australia], Melbourne. 
