New Digenetic Trematodes from Hawaiian Fishes, I 1 
Satyu Yamaguti 
This report describes thirteen new species of 
digenetic trematodes, each of which represents 
a new genus. Although some of the digenetic 
trematodes of Hawaiian fishes have been worked 
out by American authors, such as Dr. H. W. 
Manter, Dr. W. E. Martin, and Mrs. M. H. 
Pritchard, my investigations on the same group 
of parasites, carried out under a grant (GB-78) 
from the National Science Foundation, have re- 
vealed that there still are large numbers of 
undescribed species. It is really surprising that 
American authors have failed to report the 
occurrence of didymozoid trematodes, which 
are not uncommon in the Hawaiian fishes. The 
major part of the results obtained in our survey 
of the Hawaiian trematodes will be published 
in two volumes of monographs, in which every 
species found by us will be treated taxonomi- 
cally with related commentary. 
Fishes were collected at the fish market of 
Honolulu by my assistant, Mr. Shunya Kame- 
gai, and were examined by him for parasites as 
soon as possible. 
The worms were fixed just overnight under 
appropriate cover glass pressure in acetic Shau- 
dinn’s solution; on the following morning, after 
removal from the slides in water, they were re- 
fixed in a sufficient quantity of acetic Schau- 
dinn’s solution, in which they were not allowed 
to stay for more than three hours (in order to 
facilitate removal of excess mercury by treating 
with iodine in 95% alcohol). Heidenhain’s 
hematoxylin was consistently used for staining 
the specimens fixed in acetic sublimate, and 1 % 
oxalic acid, when necessary, for bleaching the 
overstained specimens, the differentiation of 
which could not be adequately controlled by 
2.5% solution of ferric ammonium sulfate. 
However, for the didymozoid trematodes which 
may be fixed with 10% formol solution, acetic 
1 Contribution No. 225, from Hawaii Marine Lab- 
oratory, University of Hawaii, Honolulu. Manuscript 
received March 16, 1964. 
formol alcohol, or acetic Schaudinn’s solution, 
with or without cover glass pressure; staining 
with Delafield’s hematoxylin ( commercial Dela- 
field’s with acetic acid added in 4%) is prefer- 
able in order to differentiate fully the male 
organs from the female organs; counterstain- 
ing with eosin is not necessary in this case. This 
method was also applied to massive trematodes 
of other families, which were, however, sub- 
jected to strong cover glass pressure by means 
of a wire compressorium. 
The figures were drafted by Mr. S. Kamegai, 
with the aid of a camera lucida for the whole 
specimens but drawn freehand for particular 
structures. They were traced and finished for 
publication by Mrs. Ikuko Yamaguti. 
The type specimens of all new species will 
be deposited in U. S. National Museum, Hel- 
minthological Collection at the Beltsville Para- 
sitological Laboratory. In this report they are 
given accession numbers ‘ (beaded by S. Y.) 
consecutive to those of the first report. 
Thanks are due to the National Science 
Foundation, Dr. G. W. Chu, Department of 
Microbiology, University of Hawaii, Dr. W. A. 
Gosline, Department of Zoology, University of 
Hawaii, my assistant, Mr. S. Kamegai, and my 
wife, Mrs. Ikuko Yamaguti. 
The new genera described herein are assigned 
to different families as follows: 
I. Lepocreadiidae Nicoll, 1935 
Bulbocirrinae n. subf. 
1. Bulb o cirrus aulostomi n. gen., n. sp. 
Lepocreadiinae Odhner, 1905 
2. Neoallolepidapedon hawaiiense 
n. gen., n. sp. 
II. Acanthocolpidae Liihe, 1909 
Acanthocolpinae Liihe, 1906 
3. Pseudacaenodera cristata 
n. gen., n. sp. 
III. Hemiuridae Liihe, 1901 
Albulatrematinae n. subf. 
4. Albulatrema ovale n. gen., n. sp. 
458 
