478 
PACIFIC SCIENCE, Vol. XIX, October 1965 
TABLE 3 
Distinguishing Features of Metanematobothrium AND Metanemato b othrioides 
CHARACTER 
M etan ematobothrium 
Metanematobothrioides 
Testes 
turning back on themselves anteriorly 
not turning back on themselves anteriorly 
Ovary 
divided 
undivided 
Receptaculum seminis 
present 
absent 
Uterus 
first descending, forming three loops 
first descending, forming one loop 
inal receptacle. Uterus proper first descending 
as far as posterior extremity, where it turns for- 
ward to be continued as ascending limb. Eggs 
bean-shaped, 18-23 X 11-14 /x. Vitellarium a 
single, slender tube running straight backward 
from genital junction to posterior extremity 
along with uterus; just before joining the germi- 
duct the vitellarium of the type is swollen into 
a bulbous reservoir 50 /x wide and distended 
with yolk cells. Excretory vesicle with terminal 
pore, bifurcating anteriorly into two short arms 
reaching to near nerve commissure. 
DISCUSSION : This new genus presents several 
features by which it can be distinguished from 
the related genus Metanematobothrium (see 
Table 3). 
Metanematobothrioides n. gen. 
GENERIC DIAGNOSIS: Didymozoidae, Nema- 
tobothriinae. Body slender, very long. Oral 
sucker well developed, followed by small phar- 
ynx. Ceca surrounded by gland cells at begin- 
ning, terminating at posterior extremity. Ace- 
tabulum distinct, some distance behind intestinal 
bifurcation. Testes paired, tubular, sinuous, ex- 
tending almost entire length of body in mature 
worms. Genital pore median, ventral to oral 
sucker. Ovary filiform, winding, unbranched, 
originating some distance behind distal ends 
of testes and descending to genital junction. No 
seminal receptacle. Uterus first descending, turn- 
ing forward at posterior extremity; eggs ellipti- 
cal, small. Vitellarium single, filiform, extend- 
ing between ovary and posterior extremity. 
Excretory vesicle with terminal pore, bifurcat- 
ing anteriorly into two short arms. Parasitic in 
marine teleosts. 
TYPE SPECIES: M. opakapaka n. sp., in Pris- 
tipomoides microlepis; Hawaii. 
12. Nematobothrioides kalikali n. gen., n. sp. 
Fig. 12 A-C 
HABITAT: Free in subcutaneous connective 
tissue of opercular region of Pristipomoides sie- 
boldii (local name "kalikali”); Hawaii. 
HOLOTYPE: U. S. Nat. Mus. Helm. Coll., S.Y. 
No. 30. 
DESCRIPTION (based on four flattened entire 
specimens and a fragmented specimen): Body 
filiform, slender, 35-70 mm long, 0.4-0. 5 mm 
wide, more or less blunt anteriorly but rounded 
at posterior extremity. Oral sucker subterminal, 
23-70 X 74-86 /x, pharynx 35-58 X 46-56 /x; 
esophagus simple, straight or sigmoid posteri- 
orly, 60-100 /x long, ceca surrounded by glandu- 
lar cells to a considerable extent at the begin- 
ning like the posterior portion of esophagus, 
terminating blindly at extreme posterior end of 
body. Acetabulum absent. 
Testes very narrow, tubular, juxtaposed, orig- 
inating one on each side a little behind genital 
junction, winding forward, turning inwards 
across ceca at about level of anterior end of 
ovary to come to lie medial to ceca, where they 
run forward parallel to each other, finally meet- 
ing in median line at a distance of 3.4 mm from 
head end in the type. From the point of union 
the vas deferens runs forward along with the 
uterus and unites with it into a short common 
duct which opens ventrally immediately behind 
the oral sucker. 
