Some Aquatic Fungi Imperfecti from Hawaii 
C J. Anastasxou 1 
Fresh-water hyphomycetes from tropical lo- 
cations have been reported by Ingold (1956, 
1958, 1959, I960), Dixon (1959), Greathead 
(1961), Hudson (1961), Hudson and Ingold 
( I960 ) , and Nilsson ( 1962 ) . Reports from the 
Pacific area include California (Ranzoni, 1953), 
and Japan (Tubaki, 1957, I960; Suzuki and 
Nimura, 1960^, b; and Nimura, I960). 
The Fungi Imperfecti reported in this paper 
were collected from streams in the Na Pali Kona 
Reserve on the island of Kauai, Hawaii, during 
August 1961. Collections were taken from the 
Kokee, Waineki, Elekiniki, Kauaikinana, and 
Kawaikoi streams, and from roadside ditches. 
At that time the streams were full and foam 
and scum were abundant. 
Most of the species reported here were identi- 
fied from spores collected in foam and scum. 
Colonies developing on rotting leaves, collected 
from the same group of streams, confirmed the 
identification of many of these fungi. The ex- 
tremely rich flora of Fungi Imperfecti included 
the following species: 
Alatospora acuminata Ingold 
Anguillospota crass a Ingold 
Anguillospora flagellifera Ingold 
Articulospora tetracladia Ingold 
Articulospora inf lata Ingold 
Campylospora chaetocladia Ranzoni 
Chaetospermum chaetosporum (Pat.) A. L. 
Smith and Ramsb. 
Clavariopsis aquatica De Wild. 
Lemonniera aquatica De Wild. 
Lunulospora curvula Ingold 
Tetrachaetum elegans Ingold 
Tricladium angulatum Ingold 
Tricladium anomalum Ingold 
Tricladium gracile Ingold 
Tricladium splendens Ingold 
Triscelophorus monosporus Ingold 
Varicosporium elodeae Kegel 
1 Department of Biology and Botany, and Faculty of 
Education, University of British Columbia, Vancouver, 
B. C. Manuscript received February 19, 1963. 
In addition to the above, vermiform spores 
similar to those of Anguillospora gigantea Ran- 
zoni, A. pseudolongissima Ranzoni, Flagello- 
spora curvula Ingold, and F. penicilliodes Ingold 
were common in scum and foam. However, 
these could not be identified with any degree 
of certainty since they did not develop on the 
leaf material observed. Monochaetia and Pesta- 
lotia spores were also very common in foam. 
Several unidentified spore types were ob- 
served, but the most common one closely re- 
sembled spores of a possible species of Articu- 
lospora illustrated by Ingold (1958:111). At 
least 50 spores of this type were observed on 
two slides made from foam collected from 
Kokee Stream. Spore size, septation, and manner 
of articulation are as described by Ingold. 
Leaves collected from the streams were plated 
out in about l A inch of distilled water. After 
about 6 weeks, tetraradiate spores developed 
abundantly above the water surface. This fungus 
produced aleuriospores consisting of an elongate, 
septate main axis continuous with the aleurio- 
phore and with elongate secondary branches 
arising from the lower part of the main axis. 
Superficially they resemble spores produced by 
species of Triscelophorus (Petersen, 1962:131- 
134). However, on the basis of the type of 
conidiophore, the morphology of the main axis 
of the spore, the manner in which the append- 
ages are produced, and the fact that there are 
always a few spores produced which lack ap- 
pendages, I have decided to consider it a species 
of Dactylella. 
Dactylella appendiculata sp. nov. 
Fungus aquaticus; mycelium septatum, hy- 
alinum, ramosum; cellulae 8-65 X 1.5-4 a; 
aleuriophori 50-400 X 1.5-4 /x, septati, hyaline 
simplices, stimmersi vel ex aqua emergentes; 
aleuriospori, apicati, hyalini, pier unique e quat 
tuor bracchiis, singillatim product!; axis princi- 
palis 57-108 (x = 84) X 9.3-14.5 /x, ex 5-8 
cellulis; bracchia divergentia septata, orientia e 
secunda cellula axis principalis, 10-136 (x = 
202 
