Materials in Evergreen Herbaceous Leaves . 
203 
Table I. 
Fall Starch and Oil Content of Leaves. 
Date. 
Material. 
Starch. 
Oils and Fats. 
Oct. 23 
Picea canadensis , Mill (B. S. P.) 
Very little 
Large amount 
„ 23 
Pyrola rotundifolia , L. 
None 
>> >» 
„ 23 
Linnaea borealis , L. var. Americana 
(Forbes), Redner 
Very little 
3 3 33 
„ 24 
Cornus canadensis , L. 
None 
33 3 ? 
„ 26 
Hypnum (sp.) 
Good reaction 
None 
„ 26 
Marchantia (sp.) 
Very little 
Large amount 
„ 26 
Mitella nuda, L. 
None 
33 33 
„ 28 
Pinus Banksiana (Lamb) 
Very little 
3 3 33 
It will be seen from the table that all the evergreens were practically 
destarchified, and on the other hand they contained a large amount of oil. 
II. Conversion of Oils and Fats to Starch. 
A series of experiments was then started to induce the formation of 
starch in the destarched plants. The following plants were brought indoors 
and subjected to higher temperatures : Linnaea , Pyrola , and Pice a. The 
first two plants were potted, but in the case of Pice a shoots were kept in 
water. The experiments were kept in a dark room at a temperature of 
68° F. (20° C.), and in each case a control was placed outside in the dark at 
the lower temperature. 
The material of Linnaea showed starch appearing as early as the 
second day, while Pyrola and Picea showed no increase, although allowed to 
remain at the higher temperature for five weeks. This points to the fact 
that different species vary in their response to the change in temperature. 
As Linnaea responded most readily to experiment, the following 
observations were all made from that plant. They are based on material 
collected and potted at intervals of five or six days during November and 
December, 1916, and January, 1917. In each case the result was based on 
a test from six to twelve leaves. 
Table II. 
Starch in Material exposed to Higher Temperature. 
Condition 
Dale. 
when 
isl day. 
2 nd day. 
jrd day. 
4 th day. 
1 week. 
potted. 
Nov. 
8 1 
experiment 
No starch 
No test 
No test 
No test 
No test 
Good reaction 
control 
>> 
>> 
33 
33 
No starch 
m j 
1 experiment 
}> 
n 
>> 
Good reaction 
33 
Strong reaction 
>» 
[ control 
n 
No starch 
33 
No starch 
.6 j 
1 experiment 
t> 
5> 
No test 
Good reaction 
Strong reaction 
| control 
if 
n 
>5 
33 
No starch 
No starch 
23 j 
1 experiment 
}> 
» 
V 
33 
Good reaction 
Strong reaction 
» 
| control 
>> 
5> 
Slight reaction 
33 
No starch 
No starch 
Dec. 
( experiment 
}} 
No starch 
33 
Good reaction 
Strong reaction 
4 , 
( control 
>> 
No reaction 
33 
No starch 
No starch 
18 ! 
1 experiment 
>5 
>> 
Slight reaction 
33 
Good reaction 
Strong reaction 
» 
j control 
>> 
y> 
No reaction 
33 
No starch 
No starch 
3 ° ; 
1 experiment 
No test 
Slight reaction 
33 
Good reaction 
Strong reaction 
V 
j control 
>> 
>» 
No reaction 
33 
No reaction 
No reaction 
