388' Wormald. — ‘ Brown Rot ’ Diseases of Fruit Trees . 
obtained in the early stages of infection in this experiment were different 
from those of previous experiments the details are given in full. 
Small particles of potatoes bearing conidiophores were cut from a pure 
culture of the fungus growing on sterilized potato and taken to the garden 
in a sterilized Petri dish, a separate dish being used for each strain. One 
of the particles was inserted, on the point of a sterilized needle, within the 
flower in such a way that 'the conidiophores came in contact with the disc 
and deposited conidia. The potato particle was then replaced in the dish 
and another taken for the next flower. Flowers were selected in which 
there was sufficient space between the stamens and ovary to allow of this 
operation being carried out without injuring the organs. 
The inoculations were made on flowers of Monarch plum trees in the 
College plantation on April i 2 ; twelve flowers were inoculated, four with 
each of the three strains. 
On April 21 no difference could be detected between the inoculated 
flowers and others in the vicinity except in the case of one flower (No. 4, 
inoculated with the cherry strain), the stamens and style of which were 
withering. Three days later, however, on most of the inoculated flowers 
there was evidence that infection had occurred. 
Strains used in inoculations. 
M. cinerea f. mali. 
Strain from a * Brown Rot ’ 
Canker of apple. 
Result , April 24. 
1. No discoloration to be detected. 
2. Slight browning on inner surface of calyx tube 
3. No discoloration. 
4. No discoloration. 
M. cinerea f. pruni. 
Strain from plum. 
1. Ovary and base of style brown ; one side of calyx tube brown 
on inner and outer surfaces. 
2. Inner surface of calyx tube brown on one side. 
3. One side of calyx tube brown on both surfaces. 
4. No discoloration. 
M. cinerea f. pruni. 1. Slight discoloration on inner surface of calyx tube. 
Strain from cherry. 2. One side of calyx tube brown on both surfaces. 
3. Slight discoloration on inner surface of calyx tube. 
4. One side of calyx tube brown on both surfaces ; style and 
stamens brown. 
When the flowers were next examined, May 7, all had been blown off 
except two, viz. No. 2 inoculated with the apple strain, and No. 3 with the 
cherry strain. The former by this time had become withered to the base 
and the disease had invaded the flowering axis and caused infection of 
a second flower (also withered) inserted there, while the latter showed 
a similar result, but in this case both infected flowers bore grey Monilia 
pustules. 
The wilting of plum flowers may be produced therefore by inoculation 
of either the stigmas or the floral disc with conidia of Monilia cinerea. 
