66 
PACIFIC SCIENCE, Vol. XX, January 1966 
TABLE 1 
Weight Changes in Animals Drained of Coelomic Fluid Before and After Being Immersed 
in 100 and 80% Sea Water for 24 Hr 
ANIMAL NO. 
INITIAL DRAINED WT 
AFTER REMOVAL 
FROM 100% 
SEA WATER 
( grams ) 
DRAINED WT 
AFTER 24 HR 
IN 100% 
SEA WATER 
(grams) 
DRAINED WT 
AFTER 24 HR 
IN 80% 
SEA WATER 
(grams) 
1 
37.4 
35.2 
2 
3L4 
29.3 
3 
39.5 
37.2 
4 
31.0 
29.2 
5 
36.4 
40.1 
6 
37.0 
39.8 
7 
33.8 
37.0 
8 
29.1 
33.7 
9 
28.2 
31.3 
over the in situ coelomic samples point to a salt 
flow from the tissue when animals are drained. 
Analysis for sodium alone was done as an index 
to the possibility of differential ion regulation 
occurring between cells and coelomic fluid as 
an aid to osmotic balance (Robertson, 1957). 
The fact that sodium concentrations are propor- 
tional to mOsmol values throughout indicates 
that ion regulation is of little importance in 
achieving osmotic equilibrium. 
Attention was given to the wet weight of tis- 
sues as a possible quantitative index of volume 
regulation during stress. The data in Table 1 
reveal a consistent pattern of tissues gaining 
TABLE 2 
Weight Measurements and Calculations Indicating Ratio of Sizes 
of Coelomic to Tissue Compartments* 
ANIMAL 
NO. 
A 
WT WITH 
COELOMIC 
FLUID 
(grams) 
B 
WT AFTER 
DRAINING 
(grams) 
C 
WT AFTER 
DESICCATION 
(grams) 
A-B 
COELOMIC 
COMPARTMENT 
(grams) 
B-C 
TISSUE 
COMPARTMENT 
(grams) 
RATIO OF 
COMPARTMENTS 
(COELOMIC: 
TISSUE) 
1 
165.2 
39.9 
2.9 
125.3 
37.0 
3.4:1 
2 
134.3 
24.0 
2.4 
114.3 
21.6 
5.1:1 
3 
156.3 
29.5 
2.7 
126.8 
26.9 
4.7:1 
4 
124.5 
33.2 
2.5 
91.3 
30.7 
3.0:1 
Average 
145.7 
31.6 
2.6 
113.1 
29.5 
3.9:1 
* One of two experiments. 
