Craspedacusta sowerbyi and Calpasoma dactyloptera — MATTHEWS 
257 
Fig. 13. Reproduction of Buchert’s figures. Row I, medusa bud ( a ) formation; Row II, atentacular hydranth 
budding; and Row 111, frustule budding. A-B and C-B indicate pathways by which tentacular hydranths are 
formed. 
The study is by no means completed. As early 
as 1939, Fuhrmann (1939:368) said, "It is very 
probable that, under favorable conditions, the 
polyp forms a medusa like Craspedacusta.” In 
light of the variability of published photographs 
and drawings of Craspedacusta medusae, this 
suggestion has real merit. 
Likewise, there is need for up-to-date, elec- 
tron microscope studies of chromosome num- 
bers in frustules. Although White (1930:230) 
states: "There seem to be twelve chromo- 
somes at each pole of late anaphase of the pri- 
mary spermatocyte," this number could not be 
verified in frustules since aceto orcein- and 
Feulgen-stained squashes (the latter, colchicine- 
pretreated) failed to reveal mitotic figures. The 
removal of cytoplasmic RNA by ribonuclease is 
now being atttempted, in the hope that only the 
DNA of the nucleus will take up the stain. 
ACKNOWLEDGMENTS 
I wish to express indebtedness to our staff 
artist Mr. Robert A. Kinzie who prepared the 
figures for publication. I wish also to acknowl- 
edge with thanks the identification of algae by 
Dr. M. S. Doty. Likewise to Dr. Voss, for infor- 
mation pertaining to the rather obscure Buchert 
paper, I am deeply grateful. I wish also to ex- 
press my appreciation to Dr. Donald Cutress, 
