68 M G Nicol. — The Bulbils and Pro-embryo of 
the plant must have been exposed to the light, and so had become green. 
With a view of confirming this I exposed tubercles to the light at the surface 
of the mud, but in no case could I obtain the formation of chlorophyll. 
Summary of Results. 
Under cultivation in jars in which there was no special provision 
for aeration, but in which the water was from time to time changed, 
L. alopecuroides has flourished vegetatively for several years, showing its 
characteristic growth and producing an abundant supply of both antheridia 
and oogonia. 
Apparently a very small number of spores are capable of germination, 
producing a pro-embryo of characteristic growth. 
The pro-embryos produced from the oospores resemble those produced 
from the underground nodes of the plant. 
In many cases the pro-embryo differs from that of other Characeae 
by the interposition of an extra oblique node, from which rhizoids are 
produced. 
In the case of the pro-embryos produced from the rhizoid-nodes 
bearing tubercles, about 30 per cent, showed this interposed node. 
For the most part reproduction takes place by means of pro-embryos, 
which are formed on the rhizoid-nodes and make use of the starch stored 
up in the tubercles. Branch pro-embryos are rare. 
Sometimes pro-embryos arise from rhizoid-nodes bearing no tubercles, 
or from the rhizoid-node of another pro-embryo. 
The tubercles either originate directly as such, or are formed by the 
transformation of rhizoids. 
The terminal rhizoid of a tubercle may again become transformed 
into a tubercle containing starch, thus forming a series of two or more 
tubercles. 
The pro-embryos arise at the basal side of the tubercle. 
Generally several pro-embryos arise from a node bearing tubercles. 
The nuclei of the tubercles are fragmented, as in the case of the 
internodal cells. 
In concluding, I desire to express my sincere thanks to Professor F. E. 
Weiss, who suggested this piece of work, and whose interest and help have 
throughout been most valuable to me. 
