the A sco carp in Lachnea ster corea , Pers . 351 
The results of these and other experiments are tabulated below : — 
No. of 
Expt. 
Medium . 
Temp. 
Time. 
Subsequent 
Treatment . 
Result. 
i(A) 
Full digestion 
38° c. 
18 hrs. 
Mounted in 
dung extract 
at 25° C. and 
cooled slowly. 
Germination 
51 hrs. after 
expt. began. 
2(B) 
33 
33 
33 
Preliminary 
changes. 
3 (C) 
Dung extract 
>9 
21 hrs. 
33 
Germination 
50 J hrs. after 
expt. began. 
4(X) 
Distilled water 
33 
4°l hrs. 
Mounted in 
water ; treated 
as above. 
No changes. 
5 00 
Dung extract 
33 
4c-| hrs. 
Mounted in ex- 
tract; treated 
as above. 
Germination 
after49| hrs. 
6 
— Na 2 Co 3 
10 2 3 
39 
31 hrs. 
Mounted in solu- 
tion used; treat- 
ed as above. 
Spores 
distorted. 
7 
N 
100 
5 > 
33 
99 
33 
Preliminary 
changes. 
8 
N 
200 
33 
33 
33 
33 
33 
9 ( 1 ) 
N 
100 
33 
39 
36 hrs. 
Mounted in solu- 
tion at 1 5 0 C. 
and cooled. 
Germination 
after 133 hrs. 
ro (II) 
j) 
39 
3 ) 
33 
Mounted in solu- 
tion at 25 0 C. 
and cooled. 
Germination 
after 181^ 
hrs. 
II (III) 
N 
200 
39 
33 
3 } 
39 
Preliminary 
changes 
i a (IV) 
Dung extract 
33 
33 
33 
33 
It is noticeable that, treated with the digestive fluids or with dung 
extract, the spores germinated in every case approximately fifty hours after 
the beginning of the experiment, and it is probable that they do so in 
nature about two days after being swallowed by the animal. Germination 
would appear to be brought about by the combined influence of warmth 
and an alkaline medium, the action of which, in part at least, is to cause 
softening of the wall of the spore. 
