Calcium and Phosphorus Metabolism of P. vigil — Sather 
195 
weights were not determined because it was not 
possible to dry thoroughly the gills and endo- 
phragmal skeleton. 
The flame spectrophotometric analysis of 
Geyer and Bowie (1961) was used to deter- 
mine the calcium content of the ashed samples. 
The blood calcium was determined using the 
method of Ferro and Ham (1957a, 1957 b). 
The method of Bernhardt, Chess, and Roy 
(1961) was used to determine the phosphorus 
(P. 2 0 5 ) content in both the ashed and blood 
samples. All flame spectrophotometric deter- 
minations were carried out on a Beckman DU 
spectrophotometer equipped with a hydrogen- 
oxygen burner and a photomultiplier. Blank 
samples were carried throughout the analysis. 
The hydration, organic, inorganic, calcium, 
and phosphorus data were transformed to arc- 
sin values and the latter were statistically ana- 
lyzed to determine whether interactions between 
the various parameters were present. The pa- 
rameters were also subjected to the D-test of 
Hartley to ascertain the differences among the 
means (Snedecor, 1959). 
RESULTS 
Table 1 contains the results of the statistical 
interaction analysis. The law of probability 
values indicate that interactions of hydration, 
organic, inorganic, calcium, and phosphorus 
contents had occurred, which illustrates that the 
chemical parameters of the organs did not uni- 
formly fluctuate from one molt stage to another. 
The interactions demonstrate that the compo- 
nents were being accumulated by the various 
TABLE 1 
Interaction Analysis of Percentage, 
Composition of Five Components 
in Sampled Organs of P. vigil 
% 
composition 
no 
F-VALUE 
PROBABILITY 
Hydration + 
16.36 
55.33 
< 0.01 
Organic 
15.78 
7.56 
< 0.01 
Inorganic 
15.98 
27.86 
< 0.01 
Calcium 
15.04 
2.86 
< 0.01 
Phosphorus 
11.30 
5.86 
< 0.01 
no = average number in each class. 
+ = calculated real values. 
fi, {2 (degrees of freedom) rr 20 and 400, respectively. 
organs during different stages, which suggests 
that the constituents were being transferred be- 
tween organs at different times. 
The results of the statistical comparisons of 
per cent hydration, inorganic and organic, and 
calcium and phosphorus among the means are 
incorporated in Tables 2, 3, and 4, respectively. 
The concentrations of the components in the 
various organs, throughout the molt cycle, are 
listed in decreasing order. In Table 2 the ap- 
pearance of a superscript number in a molt 
period signifies that the content of the organ 
at that period is greater than those with a lesser 
superscript and without a superscript. The con- 
tents during molt periods having equal super- 
scripts are not statistically different from each 
other. For example, the hydration of the mid- 
gut gland (Table 2) during the C 3 _ 4 period is 
significantly greater than that during Q. 2 , B 4 . 2 , 
Ai_ 2 , D-l. 2 , and D 3 . 4 . The amounts of mid-gut 
gland water during the C^, B 4 _ 2 , and A-^ 
periods are greater than those during D 4 _ 2 and 
D 3 _ 4 ; but, the amounts at the C 4 . 2 , B 4 . 2 , and 
A 1-2 periods do not significantly differ from 
each other. 
The ordinates of Figures 1-7 are expressed 
as per cent content, which is not the most ideal 
TABLE 2 
Comparison Among the Means: Per Cent 
Hydration of Four Organs of P. vigil 
Throughout the 
Molt Cycle 
ORGAN AND CONCENTRATION IN DECREASING 
ORDER 
mid-gut 
CARAPACE 
gill 
GLAND 
MUSCLE 
Ai-2 5 * 
C 3 
'"-'1-2 
C 3 .4 5 
c 3 - 4 4 
td 
ta 
C 3 - 4 3 
C!-2 2 
C l-2 4 
C3-4 
Bl-2 3 
Bl-2 2 
»l-2 2 
C l-2 
Al-2 1 
Ai-2 2 
Ai-2 2 
^3-4 
D l-2 
D l-2 
£*3-4 
D 4 _2 
D 3-4 
D 3-4 
D 1.2 
* Explanation of superscript numbers: 
5 = Significantly greater content than those in the last 
5 stages. 
4 = Significantly greater content than those in the last 
4 stages. 
3 : Significantly greater content than those in the last 
3 stages. 
2 = Significantly greater content than those in the last 
2 stages. 
1 - Significantly greater content than those in nonsuper- 
scripted stages. 
