326 
PACIFIC SCIENCE, VoL XXI, July 1967 
TABLE 3 ( continued ) 
PERCENTAGE 
PERCENTAGE 
OCCURRENCE 
OCCURRENCE 
IN WATER 
IN SAND 
NAME 
SAMPLES 
SAMPLES 
Didymostilbe sp. 
1.6 
Graphium sp. 
1.4 
Harpographium sp. 
1.6 
Synnematium jonesii Speare 
3.3 
1.4 
Tuberculariaceae 
Cylindrocarpon didymum (Hartung) Wo lien weber 
2.9+ 
C. radicicola Wollenweber 
1.4 
Epicoccum purpurascens Ehrenb. 
1.6 
E. sp. 
2.9 
Fusarium merismoides Corda 
1.6 
F. spp. 
22.0* 
44.7++ 
Hymenella sp. 
4.4+ 
Myrothecium roridum Tode 
3.3 
M. venue aria (Alb. and Schw.) Ditmar ex Fr. 
1.4 
M. sp. 
1.6 
1.4 
Yeasts 
Rhodotorula spp. 
27.1* 
1.4 
Orange yeasts 
1.6 
8.9++ 
Pink yeasts 
2.9+ 
Black and orange yeasts 
1.4 
Black yeasts 
11.8* 
8.9++ 
Yeasts 
45.8* 
20.8++ 
My celia sterilia (Dematiaceae) 
28.8* 
37.3++ 
My celia sterilia (Moniliaceae) 
16.9* 
40.1++ 
* Isolated from oceanic zone, 
f Isolated from the Line Islands. 
X Isolated from the Phoenix Islands. 
technique, is given in Table 1. A total of 37 
samples had fewer than 100 isolates per sam- 
ple; the remaining 30 samples had more than 
100 isolates per sample. The sand of the lee- 
ward Hawaiian islands had the highest number 
of isolates, over 500 per sample, but the num- 
ber of different species was lower than in com- 
parable samples from the Phoenix Islands. 
The sands returned from 1 to 35 species per 
sample. The majority yielded from 3 to 9 
species each. The intertidal sands of the main 
Hawaiian islands returned the highest number 
of species, a total of 68. Black Sand Beach, 
Hawaii, and Kaena Point, Oahu, each yielded 
totals of 16 species. The supratidal zone of 
Kuhio Beach, Oahu had the highest number 
of species for sites in that zone: 35 species 
among 16 genera. The zone total was 53 spe- 
cies. The subtidal zone returned the lowest 
number of species, only 22. 
In Table 2, the 18 fungi occurring most 
frequently in water and sand are listed. Among 
these, 9 are common to both areas although of 
different rank for percentage of occurrence; 
7 occur in water and not sand, and 8 occur in 
sand, not in water. Of those common to both 
water and sand, yeasts, aspergilli, and peni- 
cillia were common to all sand samples. Neither 
Rhodotorula spp. which is penultimate in rank 
for water, nor Aureobasidium pullulans, also 
frequent in water samples, was predominant 
for sand samples. 
The control plates poured when both water 
and sand samples were plated showed no 
growth. Only one colony was observed on a 
plate exposed to determine the level of air 
contamination in the laboratory. 
DISCUSSION 
Isolates obtained from water samples indi- 
cate that abundant and varied fungus popula- 
