Davis . — The Fertilization of Batrachospermum. 71 
and that the nucleus of the antherozoid need not even enter 
the trichogyne to accomplish this act of fertilization. 
V. The nucleus of the antherozoid has no fixed position in 
that structure at the time of fertilization, and frequently is 
situated at the side farthest removed from the point of fusion. 
The nucleus may never leave the antherozoid, but if the 
opening is large it may pass down into the upper portion of 
the trichogyne. Although it frequently enters the trichogyne, 
there is no particular tendency on its part to move down that 
structure, and the position of the cell-contents of the latter 
does not appear to be materially affected by its entrance. 
VI. A process of fragmentation of the nuclei of both 
antherozoid and trichogyne is very apt to begin soon after 
the fertilization of the carpogonium. 
VII. The cystocarp consists of many fertile filaments, all of 
which may be traced back to the carpogonium. When the 
communication between the trichogyne and carpogonium is 
severed, all protoplasmic union is for ever ended between the 
two structures. 
VIII. In the old trichogynes and anther ozoids one may find 
differentiated portions of the protoplasm having a faint green 
tint. These are probably the remains of the chromatophore- 
derivatives of the young antherozoids and trichogynes. Having 
a green colour, it is possible that they are functional, and that 
the long life of the trichogynes may be attributed to their 
presence. 
The idea of the trichogyne of Batrachospermum being 
a distinct cell with its own nucleus and also a chromatophore- 
derivative is quite new. From the study of certain other 
genera of Florideae, the writers upon the subject, Bornet and 
Thuret 1 , Janczewski 2 , and Schmitz 3 , have considered the 
1 Bornet et Thuret, Recherches sur la Fecondation des Floridees. Ann. d. Sci. 
Nat,, Bot., 5 e serie, t. vii, 1867. 
2 Janczewski, Notes sur le Developpement du Cystocarpe dans les Floridees. 
Mem. d. la Soc. Nat. d. Sci. Natur. d. Cherbourg, t. xx, 1877. 
3 Schmitz, Untersuchungen iiber die Befruchtung der Florideen, Sitzgb. d. k. 
Akad. d. Wiss. z. Berlin, 1883. 
