194 Phillips. — On the Development of the 
that this is the rule in the Rhodomelaceae whenever a 
superior cell is cut off at all. The considerable delay before 
the conjugation with the auxiliary cell, which takes place in 
Dasya, may indicate some amount of delay in other cases, 
and my former contention, that the superior cell was not 
usually cut off until after fertilization of the trichogyne, has 
less weight as an objection to its being considered an auxiliary 
in these cases also. 
The next figure (Fig. 5) represents the cystocarp one step 
further in development. The carpogonial branch is still 
traceable, but now the auxiliary cell has begun to form 
sporogenous filaments, represented by a dense group of cells 
seated upon the auxiliary cell. Fig. 6 shows one of these 
sporogenous filaments (shorn of lateral branches) which has 
now so developed as to outstrip the tufts of sterile filaments. 
The cells of these fertile filaments multiply doubtless with 
great rapidity. The protoplasm is densely granular, the 
nucleus deeply stained, and the pit-connexions are large and 
conspicuous. It is characteristic of the cells of these fila- 
ments that they present a concave surface upwards, into the 
well of which the pit-connexion is made. The cells of the 
sterile filaments are, on the other hand, separated from one 
another by a dense mucilage, and their pit-connexions are 
attenuated. No absorption by the auxiliary cell of the 
pericentral and other cells takes place in Dasya , as so often 
happens elsewhere. The sterile cells may be observed, though 
in an atrophied condition, even after the carpospores are already 
recognizable. Though absorption does not take place, there 
is no doubt that a large number of cells surrounding the 
central cell are put into requisition to supply the material for 
the abundant formation of carpospores, which follows upon 
the fertilization of the auxiliary cell. Among these are the 
cells of the paranematal filaments. These cells and those 
below the central cell stain deeply, and seem to be charged 
with reserve material just before the formation of spores ; 
afterwards they become vacuolated and stain feebly. 
The carpospores are ellipsoidal, and are formed in chains 
