Reproduction of Cys topics Candidas , Ldv. 307 
The cellulose layer is dissolved by long treatment with 
sulphuric acid. This is best seen in C. Candidas , where the 
projections of the surface then appear quite hollow. 
The appearance of the contents of a ripe oospore of 
C Candidas is as follows : — The large central space contains 
a globule of oily matter about half to two-thirds the diameter 
of the lumen. It is surrounded by a coarsely granular proto- 
plasm, the outer layer of which contains clear, round spots in 
variable number, three to four or more. 
In 1885 Fisch gave an account of the structure and fertiliza- 
tion in species of Pythiam , and stated that, according to his 
incomplete observations, Cyst op us appears to behave in the 
same manner. In the young oogonia of Pythiam there are 
usually from ten to twenty nuclei. In the formation of the 
oosphere these fuse together to form a single large nucleus. 
In the antheridium he always found a single nucleus, but 
thinks that this may be due to the fusion of several. The 
nucleus of the antheridium passes into the oosphere with the 
gonoplasm, and disappears there with the egg-nucleus. 
In 1888 Chmielewsky gave a description of the process 
of fertilization in C. Candidas which is very different from that 
given by Fisch. The protoplasm of the young oogonium is 
net-like, and the knots of the net, which consist of granular 
collections of plasma, stain strongly. Fisch must (says the 
author) have mistaken these knots for nuclei. In reality 
the young oogonium contains only one nucleus, and that 
is parietal. It is usually large, and has the form of an 
ellipse, in one focus of which the very small nucleolus is 
generally placed. The nucleus is very poor in chromatin, 
and stains feebly. The nucleus passes later into the centre 
of the oogonium, and the distinction between epiplasm and 
gonoplasm becomes visible. Shortly before fertilization the 
nucleus contracts a little and becomes strongly stainable (but 
even now is larger than the nuclei of the vegetative hyphae). 
The antheridium contains one nucleus only, which is similar 
in size and staining properties to the nucleus of the oosphere, 
a peculiarity which is to be noted. The passage of the male 
