308 Wager . — On the Structure and 
nucleus and gonoplasm takes place through a fertilizing tube, 
which often penetrates deeply into the oosphere. The author 
observed the two nuclei in the oosphere, and found that they 
finally become fused. The ripe oospore contains only one 
nucleus. 
Dangeard (’90) gives an account of the histology of C. can- 
didus Pers., and C. cubicus Strauss. 
In C. candidus the haustoria are very small, and contain 
only one nucleus or none at all. The nuclei in the mycelium 
are fairly well spaced. The conidia (sporangia) contain a 
hyaline protoplasm, and a number of nuclei, five to seven, as 
many as the number of zoospores which the conidia form. It 
appears that the nuclei all come from the mycelium and there 
is no division in the basidium. After the formation of a 
number of conidia, the membrane of the basidium becomes 
thick, and only two to four nuclei are left in it. 
The oogonia are generally produced at the ends of the 
branches, but may also be intercalary. They contain numerous 
nuclei which appear to possess nucleoli. The structure de- 
scribed by Chmielewsky as a single large nucleus is considered 
by Dangeard to be an oil-globule. After the differentiation of 
the protoplasm of the oogonium into oosphere and periplasm, 
a corpuscle is found in the centre of the oosphere of a variable 
form and more or less stained. In the periplasm several 
small nuclei are to be observed. Later the ripe oospore 
contains a large corpuscle in the centre, which stains strongly. 
It is nothing else than an oil-globule which is found also in 
other oospores, particularly in the Saprolegnieae and Perono- 
sporeae. Sometimes it is a sphere, sometimes a ring, some- 
times an arc of a circle. If chloroform is allowed to act long 
enough it disappears, and there is no longer any colouration. 
Sometimes when the chloroform has not acted for a sufficiently 
long time it takes the form of a sponge. 
The nuclei according to Dangeard are masked during the 
fertilization, and reappear again distinctly in the old oospore, 
in which from eight to ten nuclei can be observed placed in 
the layer of protoplasm between the globule of oil and the 
