Reproduction of Cys topics Candidas , Ldv. 319 
In the formation of oogonia, protoplasm and nuclei pass 
from the mycelium into an expansion, generally produced at 
the end of a filament, in large quantities. The protoplasm 
appears to pass in very rapidly, and there is always a dense 
mass of it at the opening leading into the young oogonium, 
and radiating from this region into the oogonium are striae 
indicating the flow or streaming of the protoplasm (Fig. 4), 
a phenomenon which Istvanffi (’ 95 ) also describes and figures. 
The oogonium at this stage often presents a contracted or 
somewhat crumpled appearance, and the protoplasm is 
arranged in an irregular network in which the foam-structure 
visible at a later stage cannot be seen. As the nuclei pass 
into the oogonium they also present a crumpled, irregular 
appearance, looking very much like knots in the protoplasm 
(Fig. 4), and it would be very easy to mistake them for such, 
if it were not that in very carefully prepared sections the 
nuclear structure can in some cases be seen. When a sufficient 
quantity of protoplasm and nuclei have passed into the 
oogonium, a transverse wall is formed separating it from the 
hypha upon which it is formed : the oogonium immediately 
expands, as if it suddenly became turgid, loses its irregular 
crumpled appearance and becomes smooth, and, so far as the 
surrounding tissues will allow, spherical in outline (Fig. 5). 
The nuclei also regain their shape and are then seen to be 
spherical in outline and similar to those in the mycelium, or 
perhaps a little larger. Even now, however, in badly prepared 
sections, it would be perfectly easy to mistake them for knots 
in the protoplasm as Chmielewsky (’88) did. The protoplasm 
at this stage has a distinct foam-structure which is very 
clearly shown in well-stained preparations, and the nuclei 
occur more or less regularly spaced in the protoplasmic 
reticulum (Fig. 6). The nuclei possess a nuclear membrane, 
a small nucleolus, and a network which is, if anything, slightly 
more distinct than in the nuclei of the mycelium. This is 
probably due to changes which appear to be set up as regards 
the supply of nutriment as soon as the oogonium becomes 
delimited from the mycelium. The number of nuclei present 
