Mutinies caninus (Muds,), Fr. 345 
Methods. 
In general, alcohol-material as needed for study was stained 
in the mass for twenty-four hours in P. Mayer’s paracarmine — 
a 70 per cent, alcoholic stain. This penetrated well. After 
washing and dehydrating, the fructifications were finally 
brought into pure cedar-oil. After they had sunk to the 
bottom in this, paraffin was gradually added to saturation. 
The fructifications were then transferred to the paraffin-bath, 
and finally imbedded in paraffin melting at 58° C. 
The sections were usually cut 6f fi thick, with a Minot- 
microtome, and were attached to the slide with Mayer’s 
albumen-fixative after floating out on the slide in water. 
The paraffin was removed with xylol. The sections were 
run down through the grades of alcohol to water, and were 
then stained on the slide with aqueous solution of safranin 
for about five minutes. 
They were mounted temporarily in water. All the drawings 
showing hyphal structure were made from preparations in 
water. They were then permanently mounted in glycerine 
by running under the cover-glass a mixture of two volumes 
of concentrated glycerine and one volume of distilled water. 
After allowing this to concentrate by evaporation from under 
the edges of the cover-glass, the mounts were cleaned and 
then sealed with hot glycerine jelly and finished with Bell's 
cement. 
Attempts were made to find staining methods which would 
give still better results than the one described, but they were 
unsuccessful in general. With very small fructifications not 
having the gelatinous layer of the volva differentiated, haema- 
toxylin stains could be used, and the dark colour which they 
gave to the walls was a great help in the camera lucida work 
of such medium power drawings as my Figs. 1 and 2. A slight 
staining of the walls of the hyphae with Ehrlich’s haematoxylin, 
just before using the safranin, may be made with advantage 
in some cases. The method of employing potassium hydrate 
with thin unattached sections is described in another place. 
A a 2 
