350 
Burt . — The Development of 
the dense portion ( 9 , by anastomosing branches or processes. 
There is great significance in the direct connexion of the 
medullary bundle M with the outer portion of the sheaf-like 
head at its base ; for it shows an original direct connexion of 
the medullary bundle with the base of all parts of the later 
plant except the outermost layer of the volva. 
Third egg. — The egg of this stage is larger, measuring 
ij mm. long by f mm. wide. By far the greater part of the 
increase in growth has been in the sheaf-like part, which now 
measured 700 fx in diameter. Differentiation of the dense 
portion 0 has now given rise to an axial column of inter- 
lacing hyphae, extending upward from the upper end m of the 
medullary bundle of Figs. 1 and 2 (R, Fig. 3). In the prepara- 
tion this column is about 500 fx long by 100 /x wide; it has 
taken an orange- red stain, and is thus quite distinct from the 
surrounding tissue on its sides ; a longitudinal direction slightly 
predominates in the position of its hyphae. This column R 
persists as an axial column of gelatinous tissue until the period 
of elongation of the stipe. 
Lying against the column R , on its sides and upper end, 
is the remainder of the dense tissue O, which is now — 
especially in its upper portion — a denser structure than R. 
It is now the region where the nuclei are most numerous 
and nearest together in the hyphae. The hyphae of this 
portion still retain their connexion with the column R ; they 
are densely interwoven and many of them are arranged 
radially. 
A gelatinous accumulation has set in at G, in the open 
tissue L. It is the beginning of the gelatinous layer of the 
volva. This gelatinous substance is regarded as a trans- 
formation-product of the hyphal wall 1 . It lies close against 
the dense portion ( 9 , in a narrow zone of irregular width — 
ranging from 20 to 80 \x in this preparation. It is stained 
orange by the safranin employed. 
1 See De Bary, Beitrage z. Morphologie u. Physiologic der Pilze, I. Reihe, (1864), 
p. 195 ; and also Van Bambeke, Sur la Morphologie du Phallus impudicus (L)., 
Bull, de la Societe Royale de Botanique de Belgique, T. XXVIII, 1889, P- 39 - • 
