360 Burt . — The Development of 
water or glycerine, even though such sections were only 6§/x 
thick. It is, however, possible to treat such thin sections so 
as to render this practicable. 
The steps of the process are as follows : — 
1. Do not attach the section to the slide. 
2. Remove the paraffin. 
3. Carefully run down through the alcohols to water. 
4. Treat for about five minutes with 7°/ o solution of potassium 
hydrate. 
5. Wash out the potassium hydrate with water. 
6 . Stain with aqueous solution of safranin or with Hoffman’s 
blue. 
7. Crush out somewhat by careful pressure on the cover 
glass. 
The process is rather long, and very delicate manipulation 
is required ; but in successful preparations the dense tissue 
opens sufficiently so that it may be drawn, and the hyphae 
separate from each other slightly so that they may be followed 
in their course with certainty. Fig. 11 has been drawn from 
such a preparation. It shows the same intricately interwoven 
structure that we have seen in the preceding stages of the 
rudiment of the stipe. This holds true also for the portion 
of the rudiment lying next to the central column R. Ed. 
Fischer 1 has, however, repeatedly figured and described this 
portion in quite as early a stage as consisting of swollen 
hyphal ends extending outwards from the central column R 
at right angles to that column, and forming a continuous 
palisade-layer along its entire length. I have made a very 
thorough search in early intermediate stages for such a 
palisade-layer, but fail to find in the region below the spore- 
bearing part any structure next to R that can be so regarded 
upon close examination. The arrangement of the protoplasm 
in the hyphae in block-like masses — which are short cells, 
perhaps — greatly increases the difficulty of determining the 
1 L. c., 1887, figs. 13 and 17 ; 1 . c , 1890, figs. 16 and 24 ; Neue Untersnch. z. 
vergleich. Entwicklungsgeschichte 11. Systematik d. Phalloideen. Denkschr. d. 
Schweiz. Naturf.-Ges., Bd. XXXIII, 1, 1893, figs. 61, 62, 72 ; 1 . c., 1895, p. 132. 
