452 Sargant . — The Formation of the. 
segments during karyokinesis in place of the usual twenty- 
four chromosomes. It is essential that any other deviations 
from the normal type of karyokinesis should be carefully 
observed and recorded, that they may be fully accounted for 
and the possibility of their masking a transverse division 
considered. For this purpose comparison with the details of 
nuclear division in the vegetative tissues of Lilinm Martagon 
is needed. Figs, i to 8 represent nuclei belonging to the 
integuments of the ovule. In the sections from which they 
are taken the embryo-sac has nearly attained its full size: 
there can be no doubt therefore that these nuclei are off the 
£ germ track/ Owing to the flattened shape of the cells, the 
karyokinetic figures are apt to be spread out in a manner 
convenient for counting the chromosomes (vid. Figs. 3 and 4). 
They are also of comparatively large size. I have compared 
other nuclei from the tissues of anthers and ovaries with these 
and find that the details of division are identical. When the 
cell is cylindrical the spindle is not flattened, and then 
resembles Fig. 9, but the separation of the chromatic 
segments takes place in just the same way. 
The structure of the resting nucleus is most clearly made 
out in tissue fixed with Flemming’s solution and stained by 
his orange method (Fig. 1). Within the nuclear membrane 
a network of fine dotted threads is partially masked by 
cloudy masses of granular substance. In alcohol preparations 
stained with methyl green and acid fuchsin this substance is 
bright green. The threads indeed in such a preparation are 
not visible at all, but the cloudy green substance is seen to be 
disposed in a coarse mesh-work. Returning to the Flemming 
preparations, it is a question whether the threads really 
anastomose with each other. They appear to do so, and 
there is always a comparatively large dark dot at the junction 
of two or more threads, but it is possible that this is an 
optical effect and that the threads only pass very closely 
behind each other. Thus the possibility of the whole net- 
work being in fact composed of one continuous much-tangled 
filament is not excluded. The whole nuclear cavity is 
