474 Sargant . — The Formation of the 
consistency for cutting. They were washed and placed suc- 
cessively in 30 c / o , 50 0 / , 7o°/ o alcohol at intervals of twenty- 
four hours, and finally removed to methylated spirit. Those 
needed for immediate embedding were left in spirit for a day 
or two, the others preserved in a mixture of about equal parts 
of alcohol, water and glycerin. The transition from water to 
spirit was always made in the dark to prevent precipitation of 
chromic acid. 
Other ovaries were placed for a couple of days in absolute 
alcohol, then transferred to methylated spirit for a week, and 
preserved in the glycerin mixture. 
B. Embedding and cutting. 
I have followed Dr. M. Heidenhaiffis embedding process 
with bergamot oil as a penetrating agent. It easily goes bad, 
and then the sections crumble under the knife. Care must be 
taken to change the oil as soon as it begins to turn yellow 
and smell rancid. I found paraffin melting at 55° C. sufficiently 
hard. 
C. Staining . 
1. Flemming’s orange method for material fixed in 
Flemming's solution. 
The sections were left about thirty hours in i c / o solution of 
safranin (Griibler’s ‘ spiritus-loslich ’) in absolute alcohol diluted 
with its own bulk of water. They were washed out in 50% 
alcohol, slightly acid, then neutral gd' / o alcohol and transferred 
to distilled water. In some cases they were placed for from 
three to five minutes in i/ o solution of potassium permanga- 
nate as a mordant, in others transferred at once to -25% 
solution of gentian violet in water. After remaining in the 
gentian violet for two to four hours they were washed out 
successively in 2 °/ o aqueous solution of Griibler’s ‘ orange G,’ 
i°/ o solution of 4 orange G’ in 5 o°/ o alcohol, and methylated 
spirit ; dehydrated, and cleared in clove oil. 
2. Renaut’s haematoxylic eosin for alcohol material. 
The sections were left all night in a solution of two to 
three drops orseillin extract diluted with 100 c.c. of water. 
