Sexual N tic lei in Li Hum Martagon. 475 
They were then rinsed and placed in a very dilute solution 
of Renaut J s haematoxylic eosin in -i°/ o aqueous solution of 
potash alum. This is useless as soon as it goes acid : great 
care was taken that it should be in good condition, violet not 
red by transmitted light. The sections may be left in twenty- 
four hours if the solution is sufficiently dilute. They were 
always washed afterwards in several changes of hard tap- 
water to keep them alkaline, and the dilute alcohols through 
which they passed were made up with tap-water. The 
orseillin stain is washed out slowly by dilute alcohol. 
EXPLANATION OF FIGURES IN PLATES 
XXII AND XXIII. 
Illustrating Miss E. Sargant’s paper on the nuclei of Lilium Martagon. 
The figures marked x 290 and X5S5 were drawn under Zeiss’ apochromatic 
hom. im. objective 2. m.m. focal length, N. A. 1.30 with eye-pieces 2 and 4 
respectively. Those marked x 1000 and x 1050 were drawn under Zeiss’ apochro- 
matic hom. im. objective 1*5 m.m. focal length, N. A. 1-30, with eye-piece 6. 
The figures were outlined with the camera lucida, and their magnification calculated 
in the usual way by projection of the scale of a stage micrometer on to the paper 
scale supplied by Zeiss. The difference between the magnifications of those 
marked x 1000 and x 1050 respectively is due to a difference in the height of the 
drawing-board on to which the outline was projected. 
Plate XXII. Lilium Martagon. 
Figs. 1-8. Vegetative nuclei from the integuments of ovules. 
Fig. 1. Resting nucleus. xiooo. 
Fig. 2. Nucleus in slender spirem. x 1000. 
Fig. 3. Nucleus just after segmentation. In this and following figure the 
chromosomes are ribbon-like, x 1000. 
Fig. 4. Early spindle, x 1000. 
Fig. 5. Eight chromosomes showing longitudinal fission from an early spindle, 
x 1000. 
Fig. 6. Three chromosomes from a nuclear plate. Separation is beginning from 
one end. x 1000. 
Fig. 7. Chromosomes from a late nuclear plate, x 1000, 
Fig. 8. Late diaster. 
