484 
Notes. 
the antherozoids to the oospheres and the first division of the spore. 
A short summary of different sets of observations on Ascophyllum is 
given in the subjoined tables. 
Series I. — Observations on Ascophyllum conducted at the Seaside. 
(a) The antherozoids were added to the oospheres at 10 o’clock a.m. 
Lot 1. Fixed 23 hours after the addition of antherozoids. Nucleus preparing for 
division. 
„ 2. „ 24 „ ,, ,, Nucleus divided, rhi- 
zoid-rudiment present, 
no dividing wall. 
,, 3&4,, 32 „ ,, „ Nucleus divided, no rhi- 
zoid, dividing wall pre- 
sent. 
,, 5. „ 36 ,, „ „ Spore divided into about 
six cells. 
(b) The antherozoids added between 11 and 12 p.m. 
Lot. 1. 
Fixed 
24 hours 
after the addition of antherozoids. 
Nucleus divided, a few 
with rhizoid-rudiments 
and division wall. 
„ 2. 
9) 
25 
yy 
yy yy 
Same result. 
0 3- 
yy 
25 
*9 
yy yy 
Not beyond spindle 
stage. 
n 4* 
yy 
28 
yy 
yy yy 
Nucleus divided, no rhi- 
zoid or dividing wall. 
Series II. — Observations on Ascophyllum carried on in the Laboratory. 
Antherozoids added between 5 and 7 p.m. 
Lot 1. Fixed 2 2| hours after the addition of antherozoids. Nucleus divided, no rhi- 
zoid or dividing wall. 
,,2. „ 23 „ „ ,, Nucleus preparing for 
division. 
,, 3* >> 23 ,, ,) ,, Same as 1. 
„ 4. „ 24I ,, ,, ,, Nucleus divided, rhizoid 
present, no dividing 
wall. 
The above observations prove that there is no essential difference 
between the behaviour of material examined in London and at the 
seaside respectively. 
After fertilisation, the cells rest for a long interval of time — com- 
monly about twenty-four hours, as shown in the foregoing table — 
