Anomuran Spermatophores — Matthews 
383 
Fig. 3. Cross section of the proximal vas deferens of 
Aniculus maximus (through region 3 of Fig. 2) showing: 
a, muscle layer; b, elongate epithelial cells; c, sectioned 
portion of arch; d, portion of wide lumen; e, foot; 
f foot-forming epithelial cells. 
Fig. 4. Cross section of the proximal vas deferens 
of Aniculus maximus (through region 4 of Fig. 2) 
showing: a , muscle layer; b, elongate epithelial cells; 
c, sectioned portion of arch; d, stalk precursor; e, foot; 
/, foot-forming epithelial cells. 
the spermatophore are here observed. Con- 
spicuous also in these sections are the longi- 
tudinal muscles (a) which probably serve to 
eject the completed spermatophores (Fig. 7). 
When the enlarged apical portions of the 
vasa deferentia of A. strigatus and A. maximus 
are placed in toluidine blue and their en- 
compassing mucus-like matrices are dissolved 
(in 0.1N KOH), continuous, non-peduncu- 
late spermatophores (Fig. 7) are revealed. 
Flere is final evidence that the arches (a) 
never completely close forming distinct am- 
pulla and that the arches are in reality never 
raised above the broad basal foot (Jb). 
DISCUSSION 
Mouchet (1931) assigns nine regions of 
activity to the vas deferens of the typical 
pedunculate spermatophore producing hermit 
crab, Diogenes pugilator Roux (Matthews, 1953 : 
264). The spermatophoric differences ob- 
served in A. strigatus and A. maximus are 
attributed to vasa deferentia which lack one 
or more of these regions. We are especially 
concerned in this discussion with region 4, in 
Fig. 5. Cross section of the proximal vas deferens 
of Aniculus maximus (through region 5 of Fig. 2) 
showing: a , muscle layer; b, elongate epithelial cells; 
c, sectioned portion of arch; d , stalk precursor; e, foot; 
/, foot-forming epithelial cells. 
which each ampulla acquires a short thick 
stalk, and with region 6, in which the stalks 
are stretched. 
