130 
W or maid . — On the Occurrence in Britain of the 
of the same fungus, inoculation experiments were carried out on trees in the 
open, using conidia obtained from cultures derived from an ascospore. 
Inoculations were made on plum flowers and fruit, cherries (fruit), and 
apple flowers. 
(a) Inoculation of Plum Flowers. 
The inoculations were made by placing conidia on the stigmas of 
fourteen flowers of Victoria plum trees in the College plantation ; the 
early symptoms of Brown Rot infection, i. e. brown discoloration of the 
stigmas and styles, were evident within four days, control flowers showing no 
such discoloration at this stage ; the ovary was killed in every case, and on 
the twelfth day after inoculation nine of the flowers had grey Monilia 
pustules on the styles, and in some cases pustules appeared on the pedicels 
and calyx. About this time a gale removed all the inoculated flowers, and 
the question as to whether the fungus can establish itself in the flowering 
spurs and twigs was not determined. The experiment shows, however, that 
the fungus is capable of killing plum flowers by infection through the 
stigmas. 
Measurements were taken of ioo conidia taken from the pedicel of one 
of the infected flowers, and the dimensions found to be io x 8*5 — 20 x 14 /x, 
average 14*8 x 108 /x. The dimensions of conidia taken from a naturally 
infected flower in another plantation were 9*5 x 7-5 — 28 x 18 /x, average 
14-5 x io-6 y.. 
(b) Inoculation of Plums {fruit). 
Conidia were placed in punctures made with a sterilized needle ; seven 
of the eight plums inoculated became infected, and within nine days bore 
many grey Monilia pustules, while in one instance infection had already 
extended to a plum in contact with one of those inoculated. * Conidia 
taken from one of the plums had dimensions of iox 8-22X 17 ju, average 
x 7*5 x 13*0 ix. 
As a control experiment eight plums were similarly punctured but not 
inoculated ; these did not become infected. 
(c) Inoculation of Cherries {fruit). 
Cherries similarly inoculated were also readily attacked ; in one 
instance pustules appeared within four days, on the rest pustules were 
observed on the sixth day. As in the case of the plums, where a sound 
cherry was in contact with an inoculated one, it became infected at the 
point of contact. Conidia taken from one of the cherries had dimensions of 
n *5 X 9 — 22*5 X 18 ix, average 17*6 x 13*2 /x. 
