Cytology of Tilletia Tritici, (Bjerk.) Wint. 
BY 
JEHANGIR FARDUNJI DASTUR, M.Sc. 
Supernumerary Mycologist , Pusa, India. 
With Plate XX and nine Figures in the Text. 
T HE investigation which forms the subject of this paper was carried 
out in the laboratory of Professor V. H. Blackman, to whom my 
acknowledgements are due for the facilities he has given and for his valuable 
help and advice. 
Bunted wheat seeds required for this work were very kindly supplied 
to me by Mr. W. L. Waterhouse, of Sydney University, to whom I am much 
indebted. These seeds were collected in Australia in November, 1918. 
My thanks are also due to Dr. S. G. Paine and Mr. R. J. Tabor, of the 
Royal College of Science, for their many acts of kindness ; and to 
Mr. J. Ramsbottom, of the British Museum, for the facilities he has given 
me in consulting books from the library. 
The cytology of Tilletia has been studied in detail by Dangeard 1 
(1892), Rawitscher 2 (1914), and Paravicini 3 (1917), and to some extent by 
Maire 4 (1902). Dangeard worked on T. caries , Tul. (=T. Tritici , (Bjerk.) 
Wint.), Rawitscher on T. Tritici , (Bjerk.) Wint., and T . levis, Kuhn, and 
Maire and Paravicini on T. Tritici. 
The spores of T. Tritici were germinated on malt extract agar. The 
germinated spores were fixed in Flemming’s weak solution. They were 
either first evenly spread on a slide smeared with egg albumen, and then 
the slide was flooded with the fixing solution ; or the germinated spores 
were first fixed in the solution and then minute drops containing the spores 
were transferred to a slide smeared with egg albumen. The fixing solution 
coagulates the egg albumen and the spores become firmly held ; both these 
1 Dangeard, P. A. : Sur la reproduction sexuelle des Champignons. Le Bot., 3® ser., 1892, 
p. 263. 
2 Rawitscher, F. : Zur Sexualitat der Brandpilze: Tilletia Tritici. Ber. d. Deut. Bot. Ges., 
vol. xxxii, 1914, p. 310. 
3 Paravicini, E. : Untersuchungen der Zellkerne bei Fortplianzung der Brandpilze. Ann. Myc., 
vol. xv, 1917, p. 75. 
1 Maire, R. : Sur la coexistence' de la nielle et de la carie dans les grains de ble. Bull. Soc. 
Myc. Fr., vol. xviii, 1902, p. 130. 
[Annals of Botany, Vol. XXXV. No. CXXXIX. July, 1921.] 
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