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ANNALES DE L’INSTITLT PASTEUR 
tozoa, which went to press early in 1912 and was published in 
Seplember of that year. !n that work 1 wroie (p. 306) : — 
« That a trypanosome or any other living cell might excrete 
grains which, when set free, could exhibit movements due to 
mob'cular or other causes, is highly probable; but that such 
grains represent a stage in ! lie iife-history of a trypanosome is 
far from being so. » At ihe time I wrote these words the only 
figure of Ihe « infeclive granules » that had been published, so 
far as I am aware, was the diagrammaiic and scarcely convin- 
cing figure given by Fry, and in my criticism I was misled by 
assuming that ihe word « granule » was used by Balfour and 
Fry in the ordinary cytological sense of the word. Subse- 
quently the formation of « infective granules » in Hæmogre- 
garines was described in detail hy Dr. Herbert Henry in 1913, 
and in the same year a full account of these bodies was 
published by Fry and Ranken. Both these memoirs were 
accompanied by numerous illustrations, from which (and spé- 
cial I y from these given by Fry and Ranken) it seems to me 
qui te clear that the term « granule » applied to these bodies is 
a complété misnomer; they are not cell-granules in the ordi- 
nary sense of the term, but endugenous buds, the formation 
of which begins by a concentration of chromidia, and each 
bud, when complété, bas the morphological and cytological 
value of a true cell, very minute in size and reduced almost 
entirely to its chromatin-elemenis ; the cytoplasm so smail in 
amount as to be practically invisible, or perhaps absent 
altogether. 
ïn order to establish this interprétation, I may first draw 
attention to the many known examples of endogenous budding 
in other Protozoa, and sprcially in the Amœbæa. There are, 
in fact, so maoy examples of this process of reproduction 
known to occur in Amœbæa that in a short note it is impos- 
sible to refer to them ail; I must content myself here by men- 
tioning a few typical instances, such as the life-history of 
ArceVa (summarized in my book, pp. 177-181 and fig. 80) and 
that of Amœba minuta , recently published by Popoff, and I wili 
deal presently in more detail with the very typical instance 
described by Liston and Martin. In Flagellata, instances of this 
metliod of reproduction are less common, but typical examples 
