u INFEGTIYES GRANULES » OF PROTOZOA 
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rent. The bud continues to grow in size and at such a stage 
as lhat figured in fig. 2, H, it only di fiers from lhe newly- 
exlruded amœba-bud (fig. 1, E) in possessing a kinetonucleus 
in addition to the princi pal nucléus. In later stages a flagell um 
grows ont lrom lhe vicinity of the kinetonucleus and the small 
tlagellate may divide, but grows finally into a trypanosome. 
Fig. 2. — Formation of endogenous buds (« infective granules »), 
in Trypanosoma rhodesiense. 
A, a trypanosome containing chromidial grains, one of which ( chr .) is 
seen coming ofï from the nucléus; B, a trypanosome containing chromidial 
grains, two of which are coming off from the surface of the body as a bud (6) ; 
G and D, trypanosomes with chromidial grains and buds (6) in process of 
libération, or libéra ted, from the body; E-L, development of the buds after 
libération from the parent; E-H, growth of the bud with formation of the 
kinetonucleus; I shows the flagellum formed; J, division of small crithidial 
forms; K and L, small trypanosomes; K, still almost crithidial in structure. 
After Fry and Ranken. 
Front the comparison of the budding in the two cases, it is 
seen that lhe bud produced by the trypanosome diflers only 
from that produced by the antœba : 1° in being very minute 
and containing very few chromidia, perhaps but a single grain 
( which, however, is of larger size in most cases than the grains 
