542 
ANNALES DE L'INSTITUT PASTEUR 
in the cytoplasm, and may be produced by a dumping and 
fusion of smaller chromidial granules) ; 2° in tbe apparent 
absence of cytoplasm. Tbe second of tbesetwo features is by 
no means uncommon in particular stages of some Protozoa, 
especially when stained by tbe Romanowsky stain, which, as 
I hâve pointed out elsewbere (1909), (ends to make tbe 
chromatin-elements appear larger than thev really are. since 
tbe red— taining substance of tbe combination deposits round 
tir* parts witch il selects as well as in them. In the micro- 
gametes of Coccidium , for example, the cytopla«mic substance 
appears to be represented only by the flagella, and in tbe 
microgameLs of the malarial parasites, when stained by tbe 
Romanowsky method, no cytoplasm can be discerned. I feel 
iustified, therefoe, in asserting that the bud formation in 
: 
trypanosomes, as described by Fry and Ranken, differs only in 
degree, and not in kind, from that already well-known to take 
place in manv amœbæ and otber Protozoa; as compared with 
the process as described by Liston and Martin, the buds of lhe 
trypanosomes give tbe impression of b<ing set free in an 
earlier and more incomplète stage of différentiation than Ihose 
of amœbæ. 
It is not necessary for me to deal in detail with lhe « gra- 
nule-shedding » described by Henry in Hæmogrrgarina simondi , 
since ibis a ut b or bas fully recognized the chromidial nature of 
these formations, and the opinion I bave expressed above with 
regard to trypanosomes applies also, mutatïs mutandis , to t lie 
case described by Henry. I may content myself by referring 
briefly to tbe. suggestions put forward by Henry to tbe effect 
that tbe « granules » are to be interpreted as recapitulating 
an older phylogenetic condition in the évolution of the Pro- 
tista. 
I hâve atways been of tbe opinion that the condition in 
which tbe chromatin-elements of the Protist bodv are in tbe 
V 
form of scattered chromidial granules was antécédent phylo- 
geneücally to the condition in which the ehromatin-grains are 
concentrated and organized into a definite nucléus. 1 imagine 
to myself the more remote ancestor of the Protozaa as a form 
witli well-developed cytoplasm containing scattered chromidial 
grains, and that from such a form the delinitely cellular cha- 
