400 Cutting. — On the Sexuality and Development of 
paper I am following the description given in Rabenhorst’s Kryptogamen 
Flora (25), which undoubtedly indicates that the fungus is A. carneus } 
Miss Welsford (27) has so recently given a summary of our knowledge 
of the development of the fruits of the Ascobolaceae as to render it unneces- 
sary for me to give a further account here. I cannot find, however, that the 
fungus Ascobolus pulcherrimus, Cr., the ascogonium of which Woronin (28) 
described, was ever named Ascophanns pulcherrimus by Crouan. This 
systematist placed the fungus in the genus Ascobolus , and since then it has 
been placed in the genus Lasiobolus by Schroter. I cannot find, in any of 
the systematic works that I have consulted, any trace of its ever being 
placed in the genus Ascophanns , although Massee (23), in his British Fungus 
Flora, has placed Lasiobolus eqidnus , an English species, in the latter genus. 
When nearly all the observations recorded in this paper were made, 
I found a reference to a paper on Ascophanns carneus , Pers., by Miss 
Ternetz (26). This paper is mostly concerned with the protoplasmic 
streaming found in the species, but it also shortly describes the fruit 
development. The account there given agrees very well indeed with what 
I have found. 
Miss Ternetz describes the archicarp as a ‘ scolecite ’, and says that it is 
very variable, both in the number and dimensions of the cells composing it, 
and also in the amount of curling of the organ. She finds no central 
cell as in Ascobolus furfuraceus and says that, in some cases, the tip of 
the ascogonium grows on so that the ascogonium becomes intercalary, and 
that it is possible that at times the portion that has grown on may form 
another ascogonium. 
She observed no copulation, and says that the wall of the young asco- 
gonium was unbroken. The cells are at first full of dense protoplasm, but 
after a time are emptied, and could not be found in full-grown apothecia. 
Methods. 
Thin slices were cut from the surface of bits of rabbit’s dung on which 
the fungus was growing. These were fixed in Flemming’s weak fluid, which 
was allowed to act for twenty-four hours. The sections were cut 4 fx or 5 /x 
in thickness and were stained either with the triple stain or with Heidenhain’s 
iron haematoxylin, and, in the latter case, sometimes counter-stained with 
erythrosin and mounted in Damar Lac. 
Germination of the Spores. 
Many attempts were made at an early period in this work to germinate 
the spores of this fungus, but these were all in vain. Agar-agar dissolved 
in rabbit’s-dung extract and acidified with orange juice was used in these 
1 I have to thank Miss A. Lorrain Smith, of the Natural History Museum, South Kensington, 
for advice on this and other systematic points. 
