402 Cutting • — On the Sexuality and Development of 
probably passing through the pore in the transverse wall, which will be 
described at the same time as the mycelium, and here it was cut off from 
the adjoining empty cells by pads of material which stopped up the pores 
in the transverse walls. 
Mycelium and Chlamydospores. 
The cells of the vegetative mycelium contain a varying quantity of proto- 
plasm and numerous nuclei (Figs. 6 and 7 ). The side walls bear a number 
of spherical granules which Miss Ternetz has already seen and described. 
These granules seem to be similar to those described in Ascobolus (Harper, 
19, Welsford, 27), Pyronema (Harper, 20 ), Humaria gramdata (Blackman 
and Fraser, 7), and other Ascomycetes. 
Harper (19) has suggested that these bodies are concerned with a trans- 
mission of materials from cell to cell, and that they are probably connected 
in some way with a pore. He does not, however, figure or describe any 
such pores in Pyronema. 
In A scop hanus car neus these granules, which vary very much in number, 
are situated on either side of a small pore in the transverse walls of the 
hyphae (Fig. 7 ). When the sections were stained with the triple stain I was 
at first unable to be absolutely certain of the presence of this pore, but after 
staining with Heidenhain’s haematoxylin and counter-staining with ery- 
throsin, There was no doubt that the pore was always present in the vegeta- 
tive hyphae, and I have since seen many well-marked pores in sections 
stained with the triple stain. 
Miss Ternetz (26) recorded that the protoplasm and vacuoles pass from 
cell to cell in this species, and suggested that there is a pore in the trans- 
verse wall. Woronin (28) has described a similar protoplasmic streaming 
in an allied form, Lasiobolus (Ascobolus) pulcherrimus. This latter species 
is also provided with granules. Neither of these authors, however, actually 
saw the pore. 
The composition of the granules and the part that they play in the 
life-history of this fungus is somewhat difficult to determine. Miss Ternetz 
examined them with microchemical tests, but was unable to come to any 
definite conclusions as to their chemical nature. She found that neither 
methylene blue nor iodine stained them, but that nuclear dyes were taken 
up with great avidity. 
I found that, although they were often stained with the safranin of the 
triple stain, they more commonly took up the gentian violet ; they had 
also the very greatest avidity for Heidenhain’s haematoxylin. I tried 
several microchemical tests as well, but was unable to form any conclusion 
as to their chemical nature. 
As to their function I can only say that under certain conditions 
