404 Cutting.- — On the Sexuality and Development of 
The ascogonium or part concerned in the formation of the ascogcnous 
hyphae is also composed of a varying number of cells from about three to 
seven. All these cells are very nearly equal in size, and there is no sign of 
the presence of any specially differentiated ‘ central cells ’ as in Ascobolus 
furfuracens. 
The terminal portion is sometimes composed of about three or five 
cells, but at other times it seems to grow on, and Miss Ternetz says that it 
is capable of growing on and forming an ascogonium, thus giving rise 
to another fruit. I have very carefully searched my preparations to see 
if I could find any trace of such a process, but have not succeeded in doing 
so. It would, however, be difficult to make sure of such a point in sections. 
The fruits often occur quite close together, and such cases were care- 
fully examined ; but neither in these, nor even in those rare cases in which 
two ascogonia are seen enclosed in one spherical pseudo-fruit-mass, was 
there any sign of continuity between two archicarps. 
It was found that a fairly large branch was in some few cases given off 
from about the middle of the ascogonium. This branch agrees in structure 
with the ascogonial region of the archicarp, and I am inclined to regard it 
merely as an ordinary branch of that region, and not as a trichogyne or an 
ascogenous hypha. 
Cytological Features. 
The cells of the archicarp, even when in an uncovered condition, are 
much larger than those of the ordinary mycelium, indeed, they are of about 
the same size as the cells of the chlamydospore-rows. The protoplasm 
of these cells is very dense and contains a few large, more or less centrally 
situated, vacuoles. These contain a number of spherical, highly refringent 
bodies with no affinity for stains. The cross-walls are perforated like those 
of the ordinary vegetative cells, and the pores are provided with the charac- 
teristic granules (Fig. 4). These cells are multinucleate, and contain many 
more nuclei than the ordinary hyphal cells (Figs. 2, 3). 
Special care was taken in the determination of the number of nuclei 
present in the cells of the young archicarp because of the claim of Harper (19), 
confirmed by Miss Welsford (27), but denied by Dangeard (10), that the 
cells of the archicarp of Ascobolus are uninucleate when first formed. No 
evidence of such a condition was obtained in Ascophanus . Very numerous 
nuclei were found in the ascogonial cells, even in the youngest of the 
uncovered ascogonia which I have seen. This question will be more fully 
discussed later. 
As the archicarp gets covered in, the cells increase in size, and the 
number of vacuoles increases. The protoplasm gets less dense and the 
nuclei become more distinct than before. Also about this time a yellow, 
irregularly spherical body appears in the middle of the ascogonial cells and 
