Relation to the Nucleolus in Gal Ionia 'candicahs , Decsne. 493 
appears to close in and to become obliterated immediately. Arrived in 
the neighbouring cell, the 4 bodies ’ remain stationary close to the wall 
(PL XXXIII, Fig. 9), so that the cell-wall is invariably seen to lie across 
the fine thread close to its junction with the body (PL XXXIII, Figs. 9, 10). 
Great caution needs to be exercised before one can admit the conviction 
that the 4 bodies ’ do come to lie in the cell adjacent to that from which 
they originated. There are two main difficulties which have to be com 
sidered. Firstly, the refractive index of the wall may be slightly different 
at the places where it has been perforated ; secondly, the walls run obliquely* 
Although these two possible sources of error have been fully appreciated, 
there seems, nevertheless, to be no other explanation than that the ‘ bodies 5 
do migrate from their parent cell into one of the neighbouring cells. 
Colouring the walls with a contrast stain to that of the nucleus, such corm 
binations as Ruthenium Red and Heidenhain, Congo Red and Heidenhain, 
Ruthenium Red and Thionin Blue, Methylene Blue and Eosin, have given 
striking and unmistakable results all confirming the foregoing conclusion. 
There is always a clear space in the cytoplasm round each c body ’ (PL 
XXXIII, Figs. 1, 4, 5 , 6 , 9, 10, and PL XXXIV, Figs. 11, 15 , 16). This 
is possibly due to an electrical condition connected with the metabolism 
proceeding in its substance. Possibly it may be compared to the space, 
which, during certain stages of nuclear division, surrounds the nucleolus. This 
possibility is strengthened by the fact that the 4 bodies ’ may be of direct 
nucleolar origin. 4 Bodies ’ belonging to the same nucleus may pass to any 
of the adjacent cells (PL XXXIII, Fig. 6), and they may lie singly or in 
groups (PI. XXXIII, Fig. 9). Thus both in transverse and in longitudinal 
sections of an anther, nuclei are seen, sending out processes and attachments 
in opposite directions. The fine connexions vary in length according to the 
distance which the 4 bodies 5 have had to travel before they enter the neigh- 
bour cell. Those that come off the knot on the side abutting on to the cell- 
wall have a short connexion (PL XXXIII, P'ig. 9), whilst those that have 
travelled in the opposite direction, and have passed through the broad area 
of cytoplasm, have a relatively long connexion (PL XXXIII, Fig. 10). At 
complete synapsis nearly every pollen mother-cell of an anther is seen to 
possess these bodies, and the threads by which they are joined to the parent 
nucleus are most conspicuous. 
Origin of 'Bodies’ from the Pre-synaptic Framework. 
The close synaptic stage is the most active time for the formation of 
4 chromatic bodies but they may often be seen to be budded off when the 
nuclear contents are being massed together prior to synapsis (PL XXXIII, 
Fig. 1). Then they can be recognized in the nuclear framework as rounded 
or oval aggregations of 4 chromatin ’ (PL XXXIII, Fig. 1). These bodies 
behave like those formed during synapsis. 
