Relation to the Nucleolus in Galtonia candicans , Dec sue. 495 
Further History of ‘Chromatic Bodies’. 
As the pollen mother-cells begin to separate from one another, the fine 
threads, connecting the ‘bodies’ with the parent nucleus, may be seen travers- 
ing the clear space in the wall. As events proceed and the walls swell, forcing 
the cells apart, the connexions are still to be seen (PI. XXXIV, Fig. 11). It 
is usual at this stage, when the nuclei are coming out of synapsis, to find the 
‘ chromatic bodies ’ lying at the extreme edge of the cytoplasm, united by 
long connecting threads to the mother-nucleus (PI. XXXIV, Figs. 15, 16). 
Whereas at synapsis nearly every nucleus has attached ‘chromatic 
bodies at the later nuclear stages only a small percentage of nuclei possess 
them. This fact indicates that the ‘ bodies ’ are gradually disintegrating. 
As the synaptic knot loosens and grows out into beaded or thready 
loops, so likewise the ‘bodies’ become granular (PI. XXXIV, Fig. 16). 
Often the connexions are in direct continuity with the loops of the spireme, 
and it is just possible that they are transferring some of their substance 
back into the parent-nucleus. The connexions are broken when the spireme 
has become more or less unravelled, and the loops are distributed through- 
out the nuclear cavity. The ‘ bodies ’ generally remain in the cytoplasm of 
the invaded cell, but sometimes they appear to be dragged across the 
space to the nucleus from which they originally came, as very occasionally 
a ‘ chromatic body ’ or a piece of one is seen adhering to the exterior of the 
nucleus. In any case the ‘ bodies ’ now disintegrate. Often portions revert 
to the eu-nucleolate condition, fragments of eu-nucleolar staining substance 
being found attached to the disorganizing ‘ chromatin ’ fragments. When 
the nucleus has returned to the centre of its surrounding cytoplasm, there is 
a sudden disappearance of the ‘ body ’ remains. The destruction must be 
extremely rapid, as in most cases at the ‘ open spireme ’ the cytoplasm 
appears homogeneous, but under favourable fixing and staining conditions 
the ‘ chromatic bodies ’ can still be recognized as brightly staining granules 
scattered in the cytoplasm (PI. XXXIV, Fig. 17). This rapid fragmenta- 
tion has its parallel in the sudden disappearance of the nucleolus when 
ejected from the equatorial plate in the somatic division. 
Origin of ‘Bodies’ from the Nucleolus. 
It is also common to find ‘bodies’ originating as buds from the nucleolus, 
but they are not given off in such numbers as from the nuclear framework. 
When newly formed at the close of the telophase of the last archesporial 
division, the nucleolus takes up a chromatin stain. As the nucleus passes 
on into the early meiotic prophase, the nucleolus gradually takes a cyto- 
plasmic stain and becomes a typical true or ‘ eu ’-nucleolus ( 20 ). It is 
especially colourless in Galtonia as compared to other plants. Chromidia 
