496 Digby.— Observations on i Chromatin Bodies' and their 
are always associated with a plasmosome of this nature. The nucleolus is 
approximately spherical, but its outline, though definite, may be undulating. 
From the early prophases onwards the nucleolus may show what 
appear to be vacuoles (PL XXXIII, Figs. 3, 5). These may be scattered 
in the nucleolus, or they may form a small group towards the centre. 
Although these nucleolar buds may be found at the early prophases, it is at 
complete synapsis that the nucleolus becomes the most active. Then it 
may be studded with buds, some in the act of breaking off (PL XXXIII, 
Fig. 9), whilst others maybe free in the nuclear cavity (PL XXXIII, Fig. 7), 
and others again may have already travelled into the surrounding cytoplasm 
(PL XXXIII, Fig. 7). Wherever they are, they instantly begin to assume 
the character of ‘ chromatic bodies \ Sometimes the buds, while still 
attached to the nucleolus, initiate this activity, and may be seen to have 
a tiny cap of ‘ chromatin ’ on their outer surfaces (PL XXXIII, Fig. 8), just 
as Page May and Walker (11) have described in the nucleolar buds of nerve 
cells of certain mammals. 
With appropriate staining a marked differentiation can be obtained 
between the small globule of nucleolar material and the angular block of 
‘ chromatin * to which ft is attached. Stained with combinations of basic 
and acid dyes, the nucleolus and the newly differentiated buds take up the 
acid stain; but as the buds get into the cytoplasm they absorb the basic dye 
in an ever increasing degree. In time the nucleolar fragments are absorbed, 
and there remains no trace of the portions of eu-nucleolus adhering to the 
‘ chromatic bodies ’. Whether the 4 bodies 5 so formed secondarily connect 
themselves with the parent-nucleus, and whether they pass through the cell- 
walls, it is impossible to say. Isolated, unconnected f bodies ’ may be often 
seen in the cytoplasm, but whether the connexions are so cut as to render 
them invisible, or whether they never existed, could not be determined. 
The nucleolus persists throughout the later nuclear stages, and is 
always in close connexion with the spireme, and later with the chromosomes, 
especially with one of the small pairs. As the chromosomes are forming, it 
begins once more to show vacuoles, and the nucleolus finally falls to 
pieces in a shower of globules (PL XXXIV, Fig. 19), much like the buds 
that were given off in the prophases and at synapsis ; but they are less 
definite at this stage of the mitosis. Some pass into the cytoplasm where 
they disappear, while others apparently become absorbed in the nucleus. 
This final destruction of the nucleolus has been recorded and figured by 
Miyake in Galtonia ( 9 ). Sometimes when the chromosomes are being 
differentiated, the nucleolus breaks up into several rounded nucleoli, each 
small nucleolus adhering to one of the paired chromosome segments 
(PL XXXIV, Fig. 18). 
