502 Digby. — Observations on ‘ Chromatin Bodies' , &c. 
EXPLANATION OF PLATES XXXIII AND XXXIV. 
Illustrating Miss L. Digby’s Observations on ‘ Chromatin Bodies ’ and their Relation to the 
Nucleolus in Galtonia candicans. 
All the figures were drawn with the camera lucida under a 2 mm. Apochr. Horn. Imm. Zeiss, 
N.A. 1.40, with Comp. Oc. 18. X2250. 
Figs. 1-11. Nuclei of pollen mother-cells. 
Figs. 12 and 13. Nuclei of megaspore mother-cells. 
Figs. 14-19. Nuclei of pollen mother-cells. 
PLATE XXXIII. 
Fig. 1. Nucleus in presynaptic stage showing ‘chromatic bodies’ being given off from the 
nuc’ear framework. Three ‘ bodies ’ have passed through the wall. 
Fig. 2. Nucleus in synapsis. Finger-like portions protruding from the synaptic knot. 
Fig. 3* Synaptic knot showing globules of nucleolar material escaping apparently independently 
of the nucleolus. 
Fig. 4. ‘Bodies’ still in the parent cell, and united to the nucleus by fine threads. 
Fig. 5. Two ‘bodies’ have passed through the wall and have entered the neighbouring cell. 
Note the ‘ chromatin ’ concentration in the substance of the ‘bodies’. 
Fig. 6. ‘ Bodies’ from the same nucleus have passed into two neighbouring cells. Two nucleoli 
present in nucleus. 
Fig. 7. Shows the origin of the ‘ bodies ’ as nucleolar buds. One bud is still attached to the 
nucleolus, one is in the clear nuclear space, while one has entered the cytoplasm. 
Fig. 8. Nucleolar buds beginning to show ‘ chromatin ’ aggregations. 
Fig. 9. Three cells showing nucleolar budding and ‘bodies ’ arranged against the wall separating 
the invaded cell from the parent cell. 
Fig. 10. Two adjacent cells with their ‘bodies’. One nucleus shows long, the other short, 
connexions. 
PLATE XXXIV. 
Fig. 11. Shows the uneven swelling of the cell-walls. The connexions joining the ‘bodies’ 
to the nucleus traverse the clear space in the wall. 
Fig. 12. Nucleus of megaspore mother-cell, in synapsis, with ‘chromatin’ staining nucleolus 
which is joined to the knot by a strand of linin. Nucleolus is vacuolated. 
Fig. 13. Synaptic knot of megaspore mother-cell with plasmosome showing buds. Insignificant 
‘ bodies ’ in nuclear cavity, and one in the neighbouring cell. 
Fig. 14. Protoplasmic connexions across the walls. 
Fig. 15. The nuclear spireme is unravelling. The cells have separated. The ‘bodies’ lie at 
the extreme edge of the cytoplasm of the invaded cell, and are still in connexion with the parent 
nucleus. 
Fig. 16. The spireme shows a beaded arrangement. There is a corresponding appearance in 
the attached ‘ bodies ’. 
Fig. 17. Nucleus in the ‘open spireme’ stage. Nucleus returned to the centre of the cell. 
The remains of the ‘bodies’ appear as bright refractive granules. Note the definite cell-wall, and 
the contraction of the cytoplasm. 
Fig. 18. The nucleolus has divided into several small nucleoli. These are attached to the 
paired chromosome segments. 
Fig. 19. The final disintegration of the nucleolus at the formation of the chromosomes. 
