Fischer.— The Biology of Ar miliaria mucida , Schrader. 527 
which was then inoculated with a piece of mycelium from a pure culture on 
bread, on which a carpophore had grown. The wound was bound up with 
a length of bast that had been previously sterilized in absolute alcohol, 
which was allowed to evaporate off before binding. As a control, neighbour 
twigs were also broken off but not inoculated. The twigs were removed on 
April 17 for examination. In one case there were no signs of infection ; in 
the other the stub of the twig broken off, half an inch in length, was dead 
and discoloured throughout, and fungal hyphae could be seen to have pene- 
trated to a depth of about one milimetre into the wood, but nowhere into 
still-living tissue. 
Four beech plants five feet high and of a rather bushy habit were pro- 
cured and planted out. One of them was inoculated in two places with 
sections of mycelium grown on bread— on the wound surface where a twig 
had been broken off, and on a cut on the stem. A control plant was 
similarly mutilated, but was not infected. 
A third bush was grafted at two points with pieces of wood permeated 
with the hyphae of A. mucida taken out of a pure culture, and a control was 
cut in the same manner, but not grafted. All four inoculation spots were 
bound with bast steeped in alcohol, and were then smeared over with 
grafting-wax. 
They were cut off on June 22, when it was found that no infection of 
living tissues had taken place. The stub left on breaking off a twig had 
died and was dry and discoloured, and in this alone had hyphae penetrated, 
but only as far as the point to which the tissues had died. 
At the end of November some beech sticks, f to 1 inch in diameter, 
were brought in from Windsor Park while still quite fresh and green. The 
cut ends were occluded with sealing-wax to hinder the evaporation of the 
moisture contained, and the sticks were inoculated with spores at places 
where the bark had been cut away sufficiently to discover the wood. They 
were then placed in a closed sterilized jar. On March 13 all were dead, no 
trace of green being revealed on incisions being made. One was cut up, but 
no signs of penetration by the hyphae could be detected. The remaining 
twigs were placed in a dish on moist earth, and on examination on May 22 
were found to be grown through and through with hyphae. 
Two other twigs, each with a side shoot and a bud attached, were 
inoculated with spores in incisions, and were placed in a jar of water. 
Early in February the buds had opened and fresh buds had formed, so that 
both twigs bore leaves, and consequently their tissues were still living. 
On March 16 one of the twigs had lost its leaves, and its tissues were found 
to be dead, but the leaves of the other were still fresh. In neither case had 
infection taken place. The inoculated spots of the dead one were then cut 
off, and the exposed surfaces were sterilized in the flame. The whole twig 
was then rubbed over with absolute alcohol, and after being allowed to dry 
