brachym meiosis 2 nd fusion TTusion- 
54 6 
Fraser and Brooks. — Further Studies on the 
areas or line of cleavage in Ascobolus. We find here a differentiation into 
dense and vacuolate areas such as is described by Faull (6), though we find 
also that the centrosome and aster are essential to the development of 
at least the denser portions. For Neotiella albocincta , Faull describes the 
spore as delimited by a ‘ curved hyaline line ' which may well correspond 
to our line of cleavage in Fig. 38 or 34. Later he distinguishes two plasma 
membranes, one of which bounds the spore-plasm, and the other the 
epiplasm in which the spore lies. This also accords with our observations. 
But while Faull does not figure an aster after spore-formation has begun, 
our investigations lead to the conclusion that the changes observed in the 
cytoplasm are due to a reaction which is set up in the neighbourhood of the 
X stercorea. 0. auraniia.P. vesiculosa. H.oranulala. Ph.corvlea. 
centrosome, and which, by producing new tensions in the ascus, eventually 
gives rise to the lines of cleavage themselves. 
In Ascobolus furfuraceus it may be suggested that the altered substance 
streaming out from the centrosome runs through or forms part of the 
hyaloplasm, and leaves the cytoplasmic reticulum comparatively unaltered ; 
the same is perhaps the case in Faull’s species. In Lachnea stercorea, on 
the other hand, the astral radiations are well marked, and this species, 
which appears to us quite comparable to Ascobolus, thus approach the 
forms studied by Harper. We have, however, obtained no evidence of 
a lateral fusion of astral rays to form a membrane such as he describes, but 
we hold rather that the rays indicate the direction of flow of altered 
Sexual asexual l 3t metaph: 2"met: 3m. S^teloph: 
