228 Barrett —Development and Sexuality of some 
Fig. 42 shows an advanced stage of gelatinization of the wall and its 
diffusion into the contents of the two cells. The nuclei of the oogonium are 
in the resting condition, while those of the antheridium are undergoing 
division. Fat globules are scattered throughout the female protoplasm, 
which is still sparse and exhibits a rather poor affinity for stain in contrast 
to that of the antheridium. 
The next condition that one observes is the beginning of the passage 
of the protoplasm of the antheridium into the oogonium. Fig. 43 represents 
a section of the sexual cells of O. luxurians stained with Gram’s stain, which 
shows this stage. It is interesting to note that the female nuclei located at 
the nodes of the protoplasmic meshes are very faint. This is due to the 
fact that, in order to bring out the male nuclei embedded in the dense, 
deeply staining protoplasm and gelatinized wall substance, it was necessary 
to decolorize to such an extent that the female nuclei lost their colour. 
The nuclei in different stages of division have different affinities for stains. 
In fact, many sections of antheridia fail to show them, while the nuclei of 
the accompanying oogonia are perfectly clear and distinct. 
There seems to be no relation between the beginning of the passage of 
the protoplasm and the condition of the nuclei. They have been observed 
in various stages of division in both cells at the time the transfer of the 
protoplasm was taking place. 
After the completion of the act of fertilization some change takes place 
in the fused protoplasm which alters its susceptibility to stain. The contents 
have a much greater affinity for safranin than previously and take up very 
little of the gentian violet. The nuclei now become distributed irregularly 
throughout the oospore in variously sized groups (Fig. 44), and are much 
smaller. The number of oil globules increases rapidly, the oogonial wall 
begins to thicken, and the communicating pore becomes closed. The nuclei 
appear under the lower powers as mere rings, but when examined with very 
high powers can be seen to possess peripherally arranged, deeply staining 
masses of chromatin (Fig. 46). They are much smaller than formerly. 
There is no apparent difference in the male and female nuclei, and this 
fact, coupled with their extremely small size, makes it impossible to definitely 
follow their subsequent relation to each other. However, there are indica- 
tions which strongly suggest a fusion of nuclei. Whether this fusion is 
always in pairs I am at present unable to say, but in the light of our present 
knowledge concerning compound fertilization this, it seems, would be assumed. 
Fig. 47 represents a portion of a section of an oospore of further develop- 
ment, The nuclei, although faint, are much larger than in previous stages 
and fewer in number. They are more regularly distributed throughout the 
interior, with a tendency to collect about the periphery. The endospore is 
now well developed. 
In Fig. 48 are shown some nuclei from oospores, highly magnified ; 
