444 
Stiles . — The Podocarpcae. 
of Podocarpus latifolius in material collected by him in two gorges near 
Cape Town on Table Mountain (Stangoolie and Orange Kloof). This 
material was mostly collected between October 18 and December 17. On 
the latter date Mr. Saxton found that the ovules in these localities had 
all aborted, so that a sequence of the later stages has not been available. 
Dr. Marie C. Stopes has also most kindly provided material of Podocarpus 
macrophyllus and P. nagi collected by her in Tokyo. This material con- 
sisted chiefly of older ovules, which provided a sequence of later stages than 
those given by the material of P. latifolius. 
I have also been enabled to examine material of Dacrydium cupres- 
sinum sent by Mr. Phillips Turner from Wellington, New Zealand, and 
material of Microcachrys tetragona collected in Tasmania and sent to 
Professor Seward by Mr. Maiden from Sydney, New South Wales. I would 
here record my hearty thanks to the botanists mentioned for their kindness 
in providing me with material, as well as to Mr. T. G. B. Osborn and 
Miss E. J. Welsford, from whom were obtained roots of Dacrydium and 
Podocarpus . I am also much indebted to the Director of the Royal Botanic 
Gardens, Kew, and to Mr. Boodle for facilities in collecting and fixing 
material. Most of the material of leaves and stems was collected at Kew, 
as well as male cones of Podocarpus andinus and female ‘ cones ’ of Dacry- 
dium Franklini. It is a pleasure to me to acknowledge my indebtedness 
to Professor V. H. Blackman for help in various ways, especially in matters 
of technique, and to Professor Seward for the trouble he has taken in helping 
to obtain material for this investigation, and for his putting at my disposal 
much literature which would otherwise have been unavailable. To my 
colleague, Mr. J. M. Hector, I am indebted for many kindnesses. 
ii. Methods. 
The methods used in this investigation require no very special comment. 
The material collected by Mr. Turner in New Zealand was preserved in 
methylated spirit ; that of Podocarpus nagi and P. macrophyllus , collected by 
Dr. Stopes at Tokyo, was preserved in dilute spirit. The material collected 
near Cape Town by Mr. Saxton was fixed in the mixture of mercuric 
chloride, picric acid, and acetic acid, as used by him in his work on the 
Callitrineae. The vegetative material collected at Kew was mostly fixed 
in methylated spirit, although some was killed in cytological fixatives. 
The young female fructifications of Dacrydium Franklini were fixed in the 
mercuric-picric-acetic mixture mentioned above, while the most suitable 
fixative for the male cones of Podocarpus andinus was found to be Flemming’s 
strong fluid. 
In the anatomical part of the work microtome series were used 
wherever possible, as well as hand sections. In a few cases it was found 
necessary to use the celloidin method. Safranin and anilin blue was found 
