3°8 
Lee. — Observations on the 
Charieis heterophylla , Cass. Seedlings smaller than in Solidago ulmi - 
folia , with anatomy correspondingly simpler, though of the same general 
type. A fairly conspicuous cotyledonary tube is present, the fusion of the 
cotyledons taking place as in Solidago. Though the vascular strands are 
very minute, the details of transition are quite clear. The following 
differences from Solidago ulmifolia may be noted : After fusing in pairs 
in the cotyledonary tube, the laterals pass inwards in the upper part of 
the hypocotyl, and on reaching the centre (Diagram 3, II and III) they 
gradually disappear or join on to the midrib bundles. In the latter, the 
xylem gradually becomes rearranged, so that quite high up in the hypocotyl 
the two protoxylems are external. The phloem part of each bundle bifur- 
cates, and the halves fuse in pairs on either side of the diarch xylem-plate 
(Diagram 3, IV). 
Aster Amelins , Linn. Seedlings small (about 2*5 cm. long), with long, 
fleshy seed-leaves and a short cotyledonary tube. The transition, which is 
not very clearly shown, is like that in Charieis heterophylla . 
Tribe IV, Inuleae. 
Inula Helenium , Linn. Seedlings small and possessing rather long, 
slender tap-roots. Transition similar to that described for Charieis 
heterophylla . 
Buphthalmum speciosum , Schreb. Seedlings small. Hypocotyl slender, 
but seed-leaves comparatively large and foliaceous, each possessing a distinct 
petiole. 
The transition is exactly as in Charieis heterophylla, with the exception 
that in the hypocotyl the intercotyledonary strands apparently disappear 
before they reach the centre. The whole of the rearrangement in the xylem 
takes place in the upper part of the hypocotyl. 
Tribe V, Heliantheae. 
Silphium perfoliatum , Linn. Seedlings large (7-12 cm. long) and 
fleshy. Seed-leaves very large, and hypocotyl stout and comparatively 
short. 
As usual in this Order, the single vascular bundle at the apex of the 
cotyledon first divides into three, after which, in correspondence with the 
large size of the cotyledons, each main strand gives off a large number of 
smaller bundles. At a lower level, these begin to re-fuse with the larger 
strands, and at the base of each cotyledon only five vascular strands remain 
— a large median one and two smaller laterals on either side (Diagram 4, i). 
In the pronounced cotyledonary tube, the extreme lateral (and smallest) 
bundles fuse with the corresponding bundles from the other cotyledon, and 
the composite structure produced, after decreasing in size, moves round 
