332 
Massee and Salmon. — Researches on 
Gliocladium penicilloides , Corda, which was observed by him closely 
associated with it, probably represented the conidial stage. In 1895, 
Matruchot ( 1 . c.) in cultivating Gliocladium penicilloides obtained 
ascigerous perithecia, which, from the description given, were clearly 
those of Eurotium insigne, Wint. Matruchot, sowing the ascospores 
obtained in this way, found that they produced a mycelium which 
gave rise to the conidiophores of the Gliocladium. In our examples, 
recorded above, on the dung of Fowls, Kangaroo, and Horse, the 
Eurotium was in each case preceded by the Gliocladium. , and we 
were able to observe clearly the organic continuity of the two forms, 
the same mycelium which produced conidiophores bearing the young 
perithecia. Culture experiments were also made, with the result of 
confirming fully Matruchot's observations. Ascospores were sown 
on sterilized films of cork floating on a decoction of dung. These 
ascospores germinated readily, producing a mycelium which at the 
end of five days gave rise to typical conidiophores of Gliocladium 
penicilloides. After eleven days the same mycelium produced peri- 
thecia (containing young asci) of Eurotium insigne. 
The Gliocladium , recorded as a new species under the name of G. 
macropodinum , by Marchal, on the dung of Kangaroo, is evidently not 
distinct from G. penicilloides , the distinguishing character relied upon, 
viz. the greater length of the conidia — ‘ 9-1 1 fx long’ instead of 
‘ 6 ^ long,’ being quite insufficient. In our specimens, on the dung 
of Kangaroo, &c., the conidia varied from 7-12 n in length ; Matruchot, 
also, expressly states that the size of the conidia is extremely variable 
in the species of Gliocladium , and gives 5-10 n as the length of the 
conidia in G. penicilloides. 
.Grove ( 1 . c.) remarks that Gliocladium differs from Penicillium in 
the spores being produced singly, not in chains ; this statement, how- 
ever, is erroneous, as in both genera the spores are originally pro- 
duced in chains, but in Gliocladium , as Matruchot points out, the 
spores soon lose this arrangement, and become irregularly massed 
in the mucilaginous head. Grove, also, referred doubtfully the 
Penicillium-Yikt fungus represented by Cooke in Plowright’s Mono- 
graph of the British Hypomyces (Grevill. xi, 49, PL 156) as occurring 
associated with Hypomyces aureo-nitens , Tub, to Gliocladium penicil- 
loides , and for this reason the statement occurs in Mass. Brit. Fung. 
FI. iii, 294, that G . penicilloides ‘is considered to be the conidial stage 
of Hypomyces aureo-nitens / It is quite clear, however, that the Fungus 
