440 Ferguson . — The Development of the Egg and 
seem to have been again resolved into granules (Fig. 18). 
Whether any of them enter the nuclear cavity and contribute 
to the formation of the achromatic spindle has not been 
definitely ascertained. The spindle, when formed, lies wholly 
within the nucleus. During the early metaphase of the 
division the nuclear membrane can still be distinguished, 
and clearly consists of a weft of threads (Figs. 20, 21). 
When the spindle arises it is ‘ multipolar in an axial plane/ 
and thus corresponds, with slight variation, to the mitotic 
figure described by Duggar (’00) in the microspore of Symplo - 
carpus foetidus , and by Wiegand (’ 99 ) in the microspore of 
Potamogeton foliosus . In Pinus , however, the upper extremi- 
ties of the threads do not at first unite into groups, but 
remain practically free, and are closely pressed against the 
neck-cells (Fig. 20). The several poles, formed at the inner 
or lower extremity of the karyokinetic figure, soon draw 
together, forming a single, very sharply defined pole ; or the 
fully developed spindle may remain more or less truncate 
at its lower end. Blackman describes this spindle as bluntly 
truncate at both extremities. I have frequently observed 
such a spindle during a late anaphase of the division, but 
this is only one of the various aspects which may be presented 
during metakinesis and later stages in this mitosis. The 
upper extremities of the achromatic spindle-fibres may never 
draw together at all ; they may unite to form two or more 
poles, or they may give rise to one pole which may be blunt 
or very slender (Figs. 22-26). But whatever form may be 
assumed by this spindle during the later stages in its develop- 
ment, there is always formed, at an early period, a diarch 
spindle (Strasburger, ’ 00 ) which is multipolar at one extremity 
and monopolar, or nearly so at the other (Figs. 20 and 21). 
A like condition also exists at an early stage in the division 
of the generative nucleus in the pines ; and it is suggested 
that such a figure may be characteristic, at least in the higher 
plants, of those indirect divisions which result in the formation 
of nuclei of different sizes. 
The chromosomes, when oriented at the nuclear plate, are 
