444 Ferguson. — The Development of the Egg and 
egg, warrants one in considering it a definite character of the 
mature oosphere. 
No cytoplasmic radiations, similar to those described by 
Belajeff (’ 91 ) in T axns baccata and by Dixon (’ 94 ) in Pinus 
sylvestris , have been observed in connexion with the fully 
developed egg-nucleus in any of the species of Pines which 
I have studied. 
During the growth and downward movement of the egg- 
nucleus, it never presents a definite network, such as is 
observed in the nucleus of the ordinary resting-cell ; but it 
is characterized at a very early stage by an open, interrupted 
reticulum, on which are arranged irregular granules of various 
sizes. This meshwork may be extremely delicate ; it may 
assume a heavy appearance ; or it may become very much 
interrupted and broken, many detached portions lying loose 
within the nuclear cavity (Figs. 27-36). Nucleoli have rarely 
been observed in this nucleus in Pinus Strobus during the 
first stages of its development (Figs. 27, 28, 30, and 31) ; but 
in P. austriaca they occasionally arise very early (Fig. 29). 
When the nucleus has attained considerable size, small nu- 
cleolus-like bodies, containing a single central vacuole, appear 
in connexion with the nuclear net ; and at the same time 
a slightly larger nucleolus is observed in the lower part of 
the nucleus, usually in connexion with its membrane (Fig. 32). 
As the nucleus continues to grow, this nucleolus also increases 
in size, gradually becoming large and very vacuolate (Figs. 
34-36)' 
When the egg-nucleus reaches maturity, it has attained 
huge dimensions, and its outline, depending on the form of the 
egg, is spherical or elliptical. The nucleolus, if demonstrable, 
is always found in the’ lower part of the nucleus; and there 
are usually several smaller bodies, designated in this paper 
as secondary nucleoli, scattered throughout the nucleus (Fig. 
36). These secondary nucleoli are invariably found in con- 
nexion with the reticulum, but, as Montgomery (’ 98 ) believed, 
they are probably caught in, not vitally united to it. They 
may be present in great abundance, or they may be entirely 
