Roots and Shoots ( Second Paper). 617 
in which the developing shoots in Series II and the developing 
roots in Series III were removed with a pointed pair of 
scissors, the water being usually changed at the same time, 
took pl^ce on April 23 and 29, May 4, 6, 10, 16, 20, 22, 25, 
and 27. 
The cultures flourished well, although some masses of 
Bacteria and some little tufts of Saprolegnia had formed 
upon the surface of the bark. The cuttings first formed 
a wound-callus at their organically upper ends, which had 
been covered with wax. Callous outgrowths were also formed 
at the lenticels. 
On May 16 I first noticed that the shoots of Series III, 
where the roots had been repeatedly removed, were less 
vigorously developed as compared with those of Series I. 
This was more conspicuous on May 20, when the leaf-blades 
of Series I were found to be, on the average, distinctly longer 
than those of Series III. 
In Series II, where the shoots had been repeatedly removed, 
a slight inferiority of development of the roots, as compared 
with those of Series I, could first be detected on May 20. In 
both series the older roots already bore lateral roots. 
On May 25, both the primary and secondary roots of 
Series I were distinctly of greater average length than 
those of Series II. Also the shoots of Series I were of 
greater average length and bore larger leaves than those of 
Series III. 
On May 31, when the experiment terminated, all the sixty- 
three cuttings were still quite healthy. Investigation showed 
that they all still contained abundant stores of starch in all 
parts. The difference in the external features of the three 
series had become more marked. In Series III only one 
shoot had attained any considerable length, whilst in Series I 
four shoots had largely grown out, and the leaf-blades of 
Series I were of greater average area than were those of 
Series III. The difference between the roots of Series I and 
II was clearly in favour of the former, though it was less 
striking than in the case of the shoots. 
